Senescence of fetal endothelial progenitor cell in pregnancy with idiopathic fetal growth

3 505

Cited 0 times in

Authors: Han Sung Hwang ; Young Guen Kwon ; Ja Young Kwon ; Yong Won Park ; Yong Sun Maeng ; Young Han Kim

Citation: JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE, Vol.25(9) : 1769-1773, 2012

MeSH: Adult ; Case-Control Studies ; Cell Differentiation/physiology ; Cell Proliferation ; Cell Separation ; Cells, Cultured ; Cellular Senescence/physiology* ; Endothelial Cells/cytology ; Endothelial Cells/pathology* ; Endothelial Cells/physiology* ; Female ; Fetal Growth Retardation/diagnostic imaging ; Fetal Growth Retardation/pathology* ; Fetal Growth Retardation/physiopathology ; Fetal Stem Cells/cytology ; Fetal Stem Cells/pathology ; Fetal Stem Cells/physiology* ; Humans ; Infant, Newborn ; Pregnancy ; Ultrasonography

Keywords: Endothelial progenitor cell ; fetal growth restriction ; senescence

Abstract: OBJECTIVE: The aim of our study was to investigate the change of count and the status of cellular senescence in fetal endothelial progenitor cells (EPCs) obtained from the umbilical cord blood of women with fetal growth restriction (FGR).

METHODS: Fetal EPCs were obtained from thirty five normal and thirty pregnant women with FGR. Each EPC was characterized and counted. EPC differentiation time and outgrowth endothelial cell (OEC) colony formation assay, senescence-associated β-galactosidase (SA-β-gal) activity assay, and telomerase activity assay were performed.

RESULTS: Fetal EPC counts were significantly decreased in the FGR group compared with normal controls. In the FGR group, the EPC differentiation time was prolonged, OEC colonies were much less formed, the staining intensity of SA-β-gal was relatively increased and the telomerase activity of EPCs was significantly decreased, compared with normal pregnancy (p < 0.001 for all).

CONCLUSIONS: The fetal EPCs in FGR pregnancies were decreased, functionally impaired and senescently altered.

Full Text: http://informahealthcare.com/doi/abs/10.3109/14767058.2012.663826

Appears in Collections:: 1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers

Yonsei Authors: Kwon, Ja Young(권자영) https://orcid.org/0000-0003-3009-6325
Kim, Young Han(김영한) https://orcid.org/0000-0003-0645-6028
Park, Yong Won(박용원)

Show full item record Find it @ YMLIB

YUHSpace: Senescence of fetal endothelial progenitor cell in pregnancy with idiopathic fetal growth