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Bfl-1/A1 Molecules are Induced in Mycobacterium Infected THP-1 Cells in the Early Time Points
DC Field | Value | Language |
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dc.contributor.author | 조상래 | - |
dc.date.accessioned | 2014-12-19T17:06:30Z | - |
dc.date.available | 2014-12-19T17:06:30Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1738-3226 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/90792 | - |
dc.description.abstract | Apoptosis is a physiological programmed cell death process. Tubercle bacilli inhibit apoptosis of alveolar macrophages and phagolysosome fusion. We investigated whether the Bcl-2 family anti-apoptotic member, Bfl-1/A1, plays an important role in the anti-apoptotic process during mycobacterial infection. PMA-treated human monocytoid THP-1 cells were infected with mycobacteria (H37Rv, BCG, and K-strain) at a multiplicity of infection (MOI) of 10 for 0, 1.5, 3, 6, 9, 12, 18, 24, 48, or 72 h. In addition, PMA-treated THP-1 cells were pretreated with specific inhibitors for 45 min before stimulation with mycobacteria at an MOI of 10 for 4 h. After the indicated time, the cells were subject to reverse transcription-polymerase chain reaction (RT-PCR) analysis, and a Bfl-1/A1-specific Western blot was performed. In PMA-differentiated THP-1 cells, the expression level of Bfl-1/A1 mRNA was increased by Mycobacterium tuberculosis (MTB) H37Rv infection. The mRNA level of Bfl-1/A1 peaked 3 h after MTB infection, then declined gradually until 9 h. However, Bfl-1/A1 mRNA induction gradually re-increased from 24 h to 72 h after MTB infection. No difference in Bfl-1/A1 expression was detected following infection with MTB H37Rv, K-strain, or M. bovis BCG. These results were not dependent on mycobacterial virulence. Moreover, mRNA levels of other anti-apoptotic molecules (Mcl-1, Bcl-2, and Bcl-xL) were not increased after MTB H37Rv or K-strain infection. These results suggest that mycobacteria induce the innate immune host defense mechanisms that utilize Bfl-1/A1 molecules at early time points, regardless of virulence. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | Journal of Experimental & Biomedcal Science (대한의생명과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Bfl-1/A1 Molecules are Induced in Mycobacterium Infected THP-1 Cells in the Early Time Points | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Park Sang-Jung | - |
dc.contributor.googleauthor | Cho Jang-Eun | - |
dc.contributor.googleauthor | Kim Yoon-Suk | - |
dc.contributor.googleauthor | Cho Sang-Nae | - |
dc.contributor.googleauthor | Lee Hye-Young | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03824 | - |
dc.relation.journalcode | J01408 | - |
dc.identifier.pmid | Apoptosis ; Mycobacterium tuberculosis ; K strain ; Bfl-1/A1 molecules | - |
dc.identifier.url | http://dbpia.co.kr/view/ar_view.asp?arid=1970951 | - |
dc.subject.keyword | Apoptosis | - |
dc.subject.keyword | Mycobacterium tuberculosis | - |
dc.subject.keyword | K strain | - |
dc.subject.keyword | Bfl-1/A1 molecules | - |
dc.contributor.alternativeName | Cho, Sang Nae | - |
dc.contributor.affiliatedAuthor | Cho, Sang Nae | - |
dc.citation.volume | 18 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 201 | - |
dc.citation.endPage | 209 | - |
dc.identifier.bibliographicCitation | Journal of Experimental & Biomedcal Science (대한의생명과학회지), Vol.18(3) : 201-209, 2012 | - |
dc.identifier.rimsid | 33540 | - |
dc.type.rims | ART | - |
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