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Discovery of candidate phospholipid biomarkers in human lipoproteins with coronary artery disease by flow field-flow fractionation and nanoflow liquid chromatography-tandem mass spectrometry

Authors
 Seul Kee Byeon  ;  Ju Yong Lee  ;  Sangsoo Lim  ;  Donghoon Choi  ;  Myeong Hee Moon 
Citation
 JOURNAL OF CHROMATOGRAPHY A, Vol.1270(28) : 246-253, 2012 
Journal Title
 JOURNAL OF CHROMATOGRAPHY A 
ISSN
 0021-9673 
Issue Date
2012
MeSH
Biomarkers/blood ; Chromatography, Liquid/methods ; Cluster Analysis ; Coronary Artery Disease/blood* ; Fractionation, Field Flow/methods* ; Humans ; Lipoproteins/blood* ; Lipoproteins/chemistry ; Phospholipids/blood* ; Principal Component Analysis ; Reproducibility of Results ; Tandem Mass Spectrometry/methods
Keywords
Lipoproteins ; HDL ; LDL ; Phospholipid ; Flow FFF ; nLC–ESI-MS–MS
Abstract
In this study, an analytical method is demonstrated to identify and develop potential phospholipid (PL) biomarkers of high density lipoprotein (HDL) and low density lipoprotein (LDL) in plasma from individuals with coronary artery disease (CAD) by employing a combination of off-line multiplexed hollow fiber flow field-flow fractionation (MxHF5) and nanoflow liquid chromatography-electrospray ionization-tandem mass spectrometry (nLC-ESI-MS-MS). HDL and LDL particles of human plasma were sorted by size at a semi-preparative scale using MxHF5, after which PL extracts of each lipoprotein fraction were qualitatively and quantitatively analyzed by nLC-ESI-MS-MS. Experiments were performed using plasma samples from 10 CAD patients and 10 controls. Quantitative analysis of the 93 PL species identified yielded a selection of 19 species from HDL fractions and 10 from LDL fractions exhibiting at least a five fold change in average concentration in CAD patients. Among the selected species, only a few were found exclusively in patient HDL fractions (18:3-LPA and 20:2/16:0-PG), control HDL fractions (16:0/16:1-PC, 20:1/20:4-PE, and 16:1-LPA), and control LDL fractions (16:0/22:3-PG). Moreover, 16:1/18:2-PC was detected from both HDL and LDL fractions of controls but disappeared in CAD patients. Although the typical change in lipoproteins for CAD is well known, with decreased levels of HDLs and reduced LDL particle size, the current study provides fundamental information on the molecular level of lipoprotein variation which can be utilized for diagnostic and therapeutic tracking.
Full Text
http://www.sciencedirect.com/science/article/pii/S0021967312017128
DOI
23195705
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Choi, Dong Hoon(최동훈) ORCID logo https://orcid.org/0000-0002-2009-9760
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90671
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