5 236

Cited 0 times in

Activation of cholera toxin production by anaerobic respiration of trimethylamine N-oxide in Vibrio cholerae

DC FieldValueLanguage
dc.contributor.author박용진-
dc.contributor.author윤미영-
dc.contributor.author윤상선-
dc.contributor.author이강무-
dc.date.accessioned2014-12-19T17:01:13Z-
dc.date.available2014-12-19T17:01:13Z-
dc.date.issued2012-
dc.identifier.issn0021-9258-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/90627-
dc.description.abstractVibrio cholerae is a gram-negative bacterium that causes cholera. Although the pathogenesis caused by this deadly pathogen takes place in the intestine, commonly thought to be anaerobic, anaerobiosis-induced virulence regulations are not fully elucidated. Anerobic growth of the V. cholerae strain, N16961, was promoted when trimethylamine N-oxide (TMAO) was used as an alternative electron acceptor. Strikingly, cholera toxin (CT) production was markedly induced during anaerobic TMAO respiration. N16961 mutants unable to metabolize TMAO were incapable of producing CT, suggesting a mechanistic link between anaerobic TMAO respiration and CT production. TMAO reductase is transported to the periplasm via the twin arginine transport (TAT) system. A similar defect in both anaerobic TMAO respiration and CT production was also observed in a N16961 TAT mutant. In contrast, the abilities to grow on TMAO and to produce CT were not affected in a mutant of the general secretion pathway. This suggests that V. cholerae may utilize the TAT system to secrete CT during TMAO respiration. During anaerobic growth with TMAO, N16961 cells exhibit green fluorescence when stained with 2',7'-dichlorofluorescein diacetate, a specific dye for reactive oxygen species (ROS). Furthermore, CT production was decreased in the presence of an ROS scavenger suggesting a positive role of ROS in regulating CT production. When TMAO was co-administered to infant mice infected with N16961, the mice exhibited more severe pathogenic symptoms. Together, our results reveal a novel anaerobic growth condition that stimulates V. cholerae to produce its major virulence factor.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJOURNAL OF BIOLOGICAL CHEMISTRY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAmino Acid Substitution-
dc.subject.MESHAnaerobiosis/drug effects-
dc.subject.MESHAnaerobiosis/genetics-
dc.subject.MESHAnimals-
dc.subject.MESHBacterial Secretion Systems/drug effects-
dc.subject.MESHBacterial Secretion Systems/physiology*-
dc.subject.MESHCholera/enzymology*-
dc.subject.MESHCholera/genetics-
dc.subject.MESHCholera Toxin/genetics-
dc.subject.MESHCholera Toxin/secretion*-
dc.subject.MESHMethylamines/pharmacology-
dc.subject.MESHMice-
dc.subject.MESHMutation, Missense-
dc.subject.MESHOxidants/pharmacology-
dc.subject.MESHPeriplasm/genetics-
dc.subject.MESHPeriplasm/secretion*-
dc.subject.MESHVibrio cholerae/enzymology*-
dc.subject.MESHVibrio cholerae/genetics-
dc.subject.MESHVibrio cholerae/pathogenicity-
dc.subject.MESHVirulence Factors/genetics-
dc.subject.MESHVirulence Factors/metabolism*-
dc.titleActivation of cholera toxin production by anaerobic respiration of trimethylamine N-oxide in Vibrio cholerae-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Microbiology (미생물학)-
dc.contributor.googleauthorKang-Mu Lee-
dc.contributor.googleauthorYongjin Park-
dc.contributor.googleauthorWasimul Bari-
dc.contributor.googleauthorMi Young Yoon-
dc.contributor.googleauthorJunhyeok Go-
dc.contributor.googleauthorSang Cheol Kim-
dc.contributor.googleauthorHyung-il Lee-
dc.contributor.googleauthorSang Sun Yoon-
dc.identifier.doi23019319-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02549-
dc.contributor.localIdA02558-
dc.contributor.localIdA02638-
dc.contributor.localIdA01584-
dc.relation.journalcodeJ01258-
dc.identifier.eissn1083-351X-
dc.identifier.pmid23019319-
dc.identifier.urlhttp://www.jbc.org/content/287/47/39742.long-
dc.subject.keywordBacterial Pathogenesis-
dc.subject.keywordBacterial Toxins-
dc.subject.keywordCholera Toxin-
dc.subject.keywordRespiration-
dc.subject.keywordVirulence Factors-
dc.subject.keywordAnaerobic Respiration-
dc.subject.keywordTrimethylamine N-Oxide-
dc.subject.keywordVibrio Cholerae-
dc.contributor.alternativeNamePark, Yong Jin-
dc.contributor.alternativeNameYoon, Mi Young-
dc.contributor.alternativeNameYoon, Sang Sun-
dc.contributor.alternativeNameLee, Kang Mu-
dc.contributor.affiliatedAuthorYoon, Mi Young-
dc.contributor.affiliatedAuthorYoon, Sang Sun-
dc.contributor.affiliatedAuthorLee, Kang Mu-
dc.contributor.affiliatedAuthorPark, Yong Jin-
dc.citation.volume287-
dc.citation.number47-
dc.citation.startPage39742-
dc.citation.endPage39752-
dc.identifier.bibliographicCitationJOURNAL OF BIOLOGICAL CHEMISTRY, Vol.287(47) : 39742-39752, 2012-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.