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Corticotropin-releasing hormone downregulates IL-10 production by adaptive forkhead box protein 3-negative regulatory T cells in patients with atopic dermatitis

Authors
 Sang Ho Oh  ;  Chang Ook Park  ;  Wen Hao Wu  ;  Ji Young Kim  ;  Shan Jin  ;  Dashlkhumbe Byamba  ;  Byung Gi Bae  ;  Seongmin Noh  ;  Beom Jin Lim  ;  Ji Yeon Noh  ;  Kwang Hoon Lee 
Citation
 JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, Vol.129(1) : 151-159, 2012 
Journal Title
 JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY 
ISSN
 0091-6749 
Issue Date
2012
MeSH
Adolescent ; Adult ; Corticotropin-Releasing Hormone/pharmacology* ; Cytokines/biosynthesis ; Dermatitis, Atopic/genetics ; Dermatitis, Atopic/immunology* ; Dermatitis, Atopic/metabolism ; Down-Regulation/drug effects ; Female ; Forkhead Transcription Factors/metabolism* ; Gene Expression Regulation/drug effects ; Humans ; Interleukin-10/biosynthesis* ; Male ; RNA, Messenger ; Receptors, Corticotropin-Releasing Hormone/genetics ; Receptors, Corticotropin-Releasing Hormone/metabolism ; T-Lymphocytes, Regulatory/drug effects* ; T-Lymphocytes, Regulatory/immunology* ; Th1 Cells/drug effects ; Th1 Cells/immunology ; Th2 Cells/drug effects ; Th2 Cells/immunology ; Young Adult
Keywords
Corticotropin-releasing hormone ; stress ; atopic dermatitis ; IL-10 ; forkhead box protein 3 ; regulatory T cell
Abstract
BACKGROUND: Corticotropin-releasing hormone (CRH) is the central regulating hormone of the hypothalamic-pituitary-adrenal axis. CRH also has diverse functional effects in the periphery and is related to the aggravation of several cutaneous diseases; however, the effect of CRH on T cells in patients with atopic dermatitis (AD) has not been well evaluated. OBJECTIVE: We investigated whether CRH directly affects peripheral T(H)1, T(H)2, and regulatory T (Treg) cells in patients with AD. METHODS: We assessed whether T cells express the CRH receptor protein and mRNA by using flow cytometry, Western blotting, immunofluorescence, immunohistochemistry, and RT-PCR. We evaluated cytokine expression using ELISA after treating the T cells extracted from patients with AD and healthy control subjects (HCs) with CRH. Flow cytometry was then used to evaluate any direct effects of CRH on T(H)1, T(H)2, and Treg cells from patients with AD and HCs. RESULTS: T cells from patients with AD expressed significantly lower CRH receptor 1/2 mRNA levels than T cells from HCs. T cells from HCs reacted with different IL-4 and IFN-γ secretions to CRH treatment, whereas T cells from patients with AD did not. IL-10 production was significantly decreased in the supernatants from both the HCs and patients with AD after CRH treatment. CRH upregulated IL-4 production by T(H)2 cells and downregulated IFN-γ production by T(H)1 cells in HCs. CRH also suppressed the production of IL-10 by forkhead box protein 3-negative Treg cells in both groups, but the difference was only significant in patients with AD. CONCLUSIONS: CRH-mediated suppression of IL-10 secretion from Treg cells might explain stress-related exacerbations in patients with AD.
Full Text
http://www.sciencedirect.com/science/article/pii/S0091674911014539
DOI
22000570
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Park, Chang Ook(박창욱) ORCID logo https://orcid.org/0000-0003-3856-1201
Oh, Sang Ho(오상호) ORCID logo https://orcid.org/0000-0002-4477-1400
Lee, Kwang Hoon(이광훈)
Lim, Beom Jin(임범진) ORCID logo https://orcid.org/0000-0003-2856-0133
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90606
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