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Function of the Cold Receptor (TRPM8) Associated with Voiding Dysfunction in Bladder Outlet Obstruction in Rats.

DC Field Value Language
dc.contributor.author강효진-
dc.contributor.author김매화-
dc.contributor.author이혜영-
dc.contributor.author임영재-
dc.contributor.author전지희-
dc.contributor.author정현진-
dc.contributor.author한상원-
dc.date.accessioned2014-12-19T16:58:55Z-
dc.date.available2014-12-19T16:58:55Z-
dc.date.issued2012-
dc.identifier.issn2093-4777-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/90557-
dc.description.abstractPURPOSE: Bladder outlet obstruction (BOO) causes storage and voiding dysfunction in the lower urinary tract. We investigated the expression of transient receptor potential cation channel subfamily M member 8 (TRPM8) to evaluate the relationship between TRPM8 expression and overactive bladder (OAB) in a rat model of BOO. METHODS: Fifty female Sprague-Dawley rats were divided into 4 groups; normal (n=10), normal-menthol (n=10), BOO (n=15), BOO-menthol (n=15). After 3 weeks, cystometry was performed by infusing physiological saline and menthol (3 mM) into the bladder at a slow infusion rate. The histological changes and expression of TRPM8 in the bladder were investigated by Masson's trichrome staining, immunofluorescence and reverse transcription-polymerase chain reaction. RESULTS: Cystometry showed that the intercontraction interval (ICI; 428.2±23.4 vs. 880.4±51.2, P<0.001), micturition pressure (MP; 25.7±1.01 vs. 71.80±3.01, P<0.001), and threshold pressure (2.9±0.25 vs. 9.2±1.58, P<0.01) were significantly increased in BOO rats. The bladder wall was significantly dilated compared with the control. Detrusor muscle hypertrophy and a thick mucosa layer were observed in BOO bladder. After menthol treatment, ICIs were decreased and MPs were increased in the menthol treatment groups. TRPM8-positive cells and mRNA were predominantly increased in the bladder and dorsal root ganglia of all groups compared with the normal group. CONCLUSIONS: Increased bladder wall thickness and proportion of collagen probably affect voiding dysfunction. Furthermore, an increase of TRPM8 expression in BOO may induce entry of Ca(2+) from the extracellular space or stores. The increase of Ca(2+) probably causes contraction of smooth muscle in BOO. However, OAB symptoms were not observed after menthol treatment although the expression of TRPM8 was abundant in the bladder epithelium after menthol treatment. Although OAB in BOO models may be caused by complex pathways, regulation of TRPM8 presents possibilities for OAB treatment.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfINTERNATIONAL NEUROUROLOGY JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleFunction of the Cold Receptor (TRPM8) Associated with Voiding Dysfunction in Bladder Outlet Obstruction in Rats.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.googleauthorJi Hee Jun-
dc.contributor.googleauthorHyo Jin Kang-
dc.contributor.googleauthorMei Hua Jin-
dc.contributor.googleauthorHye Young Lee-
dc.contributor.googleauthorYoung Jae Im-
dc.contributor.googleauthorHyun Jin Jung-
dc.contributor.googleauthorSang Won Han-
dc.identifier.doi22816047-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA04285-
dc.contributor.localIdA03316-
dc.contributor.localIdA00101-
dc.contributor.localIdA00421-
dc.contributor.localIdA03387-
dc.contributor.localIdA03553-
dc.contributor.localIdA03772-
dc.relation.journalcodeJ01171-
dc.identifier.eissn2093-6931-
dc.identifier.pmid22816047-
dc.subject.keywordBladder outlet obstruction-
dc.subject.keywordCold receptor-
dc.subject.keywordMenthol-
dc.subject.keywordTRPM8-
dc.contributor.alternativeNameKang, Hyo Jin-
dc.contributor.alternativeNameJin, Mei Hua-
dc.contributor.alternativeNameLee, Hye Young-
dc.contributor.alternativeNameIm, Young Jae-
dc.contributor.alternativeNameJun, Ji Hee-
dc.contributor.alternativeNameJung, Hyun Jin-
dc.contributor.alternativeNameHan, Sang Won-
dc.contributor.affiliatedAuthorHan, Sang Won-
dc.contributor.affiliatedAuthorLee, Hye Young-
dc.contributor.affiliatedAuthorKang, Hyo Jin-
dc.contributor.affiliatedAuthorJin, Mei Hua-
dc.contributor.affiliatedAuthorIm, Young Jae-
dc.contributor.affiliatedAuthorJun, Ji Hee-
dc.contributor.affiliatedAuthorJung, Hyun Jin-
dc.citation.volume16-
dc.citation.number2-
dc.citation.startPage69-
dc.citation.endPage76-
dc.identifier.bibliographicCitationINTERNATIONAL NEUROUROLOGY JOURNAL, Vol.16(2) : 69-76, 2012-
dc.identifier.rimsid32832-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Urology (비뇨의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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