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Vitamin B12-mediated restoration of defective anaerobic growth leads to reduced biofilm formation in Pseudomonas aeruginosa.

DC Field Value Language
dc.contributor.author용동은-
dc.contributor.author윤상선-
dc.contributor.author이강무-
dc.contributor.author김상철-
dc.contributor.author박용진-
dc.date.accessioned2014-12-19T16:54:11Z-
dc.date.available2014-12-19T16:54:11Z-
dc.date.issued2012-
dc.identifier.issn0019-9567-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/90408-
dc.description.abstractPseudomonas aeruginosa undergoes cell elongation and forms robust biofilms during anaerobic respiratory growth using nitrate (NO(3)(-)) as an alternative electron acceptor. Understanding the mechanism of cell shape change induced upon anaerobiosis is crucial to the development of effective treatments against P. aeruginosa biofilm infection. Here, we uncovered the molecular basis of anaerobiosis-triggered cell elongation and identified vitamin B(12) to be a molecule that can reinstate defective anaerobic growth of P. aeruginosa. The ratio of total cellular DNA content to protein content was significantly decreased in the PAO1 strain grown under anaerobic conditions, indicating that DNA replication is impaired during anaerobic growth. Anaerobic growth of PAO1 reached a higher cell density in the presence of vitamin B(12), an essential coenzyme of class II ribonucleotide reductase. In addition, cell morphology returned to a normal rod shape and transcription of stress-response genes was downregulated under the same anaerobic growth conditions. These results suggest that vitamin B(12), the production of which was suppressed during anaerobic growth, can restore cellular machineries for DNA replication and therefore facilitate better anaerobic growth of P. aeruginosa with normal cell division. Importantly, biofilm formation was substantially decreased when grown with vitamin B(12), further demonstrating that anaerobiosis-induced cell elongation is responsible for robust biofilm formation. Taken together, our data reveal mechanistic details of a morphological change that naturally occurs during anaerobic growth of P. aeruginosa and illustrates the ability of vitamin B(12) to modulate the biofilm-forming capacity of P. aeruginosa under such condition.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfINFECTION AND IMMUNITY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAerobiosis-
dc.subject.MESHAnaerobiosis/drug effects-
dc.subject.MESHBiofilms/growth & development*-
dc.subject.MESHDNA, Bacterial/metabolism-
dc.subject.MESHGene Expression Profiling-
dc.subject.MESHGene Expression Regulation, Bacterial/physiology-
dc.subject.MESHOxygen Consumption-
dc.subject.MESHProtein Array Analysis-
dc.subject.MESHPseudomonas aeruginosa/genetics*-
dc.subject.MESHPseudomonas aeruginosa/physiology*-
dc.subject.MESHRibonucleotide Reductases/genetics-
dc.subject.MESHRibonucleotide Reductases/metabolism-
dc.subject.MESHVitamin B 12/metabolism*-
dc.subject.MESHVitamin B Complex/pharmacology*-
dc.titleVitamin B12-mediated restoration of defective anaerobic growth leads to reduced biofilm formation in Pseudomonas aeruginosa.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthorKang-Mu Lee-
dc.contributor.googleauthorJunhyeok Go-
dc.contributor.googleauthorMi Young Yoon-
dc.contributor.googleauthorYongjin Park-
dc.contributor.googleauthorSang Cheol Kim-
dc.contributor.googleauthorDong Eun Yong-
dc.contributor.googleauthorSang Sun Yoon-
dc.identifier.doi22371376-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02423-
dc.contributor.localIdA02558-
dc.contributor.localIdA02638-
dc.contributor.localIdA00529-
dc.contributor.localIdA01584-
dc.relation.journalcodeJ01055-
dc.identifier.eissn1098-5522-
dc.identifier.pmid22371376-
dc.subject.keywordAerobiosis-
dc.subject.keywordAnaerobiosis/drug effects-
dc.subject.keywordBiofilms/growth & development*-
dc.subject.keywordDNA, Bacterial/metabolism-
dc.subject.keywordGene Expression Profiling-
dc.subject.keywordGene Expression Regulation, Bacterial/physiology-
dc.subject.keywordOxygen Consumption-
dc.subject.keywordProtein Array Analysis-
dc.subject.keywordPseudomonas aeruginosa/genetics*-
dc.subject.keywordPseudomonas aeruginosa/physiology*-
dc.subject.keywordRibonucleotide Reductases/genetics-
dc.subject.keywordRibonucleotide Reductases/metabolism-
dc.subject.keywordVitamin B 12/metabolism*-
dc.subject.keywordVitamin B Complex/pharmacology*-
dc.contributor.alternativeNameYong, Dong Eun-
dc.contributor.alternativeNameYoon, Sang Sun-
dc.contributor.alternativeNameLee, Kang Mu-
dc.contributor.alternativeNameKim, Sang Cheol-
dc.contributor.alternativeNamePark, Yong Jin-
dc.contributor.affiliatedAuthorYong, Dong Eun-
dc.contributor.affiliatedAuthorYoon, Sang Sun-
dc.contributor.affiliatedAuthorLee, Kang Mu-
dc.contributor.affiliatedAuthorKim, Sang Cheol-
dc.contributor.affiliatedAuthorPark, Yong Jin-
dc.citation.volume80-
dc.citation.number5-
dc.citation.startPage1639-
dc.citation.endPage1649-
dc.identifier.bibliographicCitationINFECTION AND IMMUNITY, Vol.80(5) : 1639-1649, 2012-
dc.identifier.rimsid34198-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers

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