Cited 1 times in
Metabolic effects of a stabilizing peptide fusion protein of leptin in normal mice
DC Field | Value | Language |
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dc.contributor.author | 박현주 | - |
dc.contributor.author | 이샛별 | - |
dc.contributor.author | 김종선 | - |
dc.date.accessioned | 2014-12-19T16:53:04Z | - |
dc.date.available | 2014-12-19T16:53:04Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0018-5043 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/90375 | - |
dc.description.abstract | Leptin is a protein hormone produced by adipocytes. It is secreted into the blood stream and plays a key role in regulating body energy homeostasis by inhibiting feeding behavior followed by decreased body weight. Because protein aggregation is a major problem in therapeutic proteins, we previously demonstrated that a stabilizing peptide (SP) fusion protein of leptin (SP-leptin) appeared to resist aggregation induced by agitation, freezing/thawing, or heat stress. In this study, we fused mouse leptin with the stabilizing peptide and compared the biological activities of leptin and SP-leptin in vivo using a male C57Bl mouse model and ex vivo using MCF7 breast cancer cell lines. Each group of mice was treated with saline, leptin, and SP-leptin for 20 days and the differences in body weight, food intake, abdominal fat contents, and TG concentration were measured. The SP-leptin appeared to decrease the body weight and food intake in male C57Bl mice more significantly than wild type leptin, and the SP-leptin treated MCF7 cells displayed better cell proliferation than leptin. As a consequence of decreased body weight, the SP-leptin treated mouse group showed decreased abdominal fat contents and low triglyceride (TG) concentration. Moreover, the SP-leptin treated mouse group had fewer lipid droplets in liver and reduced lipid droplet size when analyzed by Oil red O and H & E staining. These results demonstrated that SP-leptin is more effective than wild type leptin in normal mice in lowering their body weight and fat contents in the abdominal region, the serum, and the liver. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | HORMONE AND METABOLIC RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Abdominal Fat/drug effects | - |
dc.subject.MESH | Adiposity/drug effects | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Body Weight/drug effects | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Feeding Behavior/drug effects | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Leptin/pharmacology* | - |
dc.subject.MESH | Lipids/blood | - |
dc.subject.MESH | Liver/drug effects | - |
dc.subject.MESH | Liver/metabolism | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Metabolic Networks and Pathways/drug effects* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Peptides/pharmacology* | - |
dc.subject.MESH | Protein Stability/drug effects | - |
dc.subject.MESH | Recombinant Fusion Proteins/pharmacology* | - |
dc.title | Metabolic effects of a stabilizing peptide fusion protein of leptin in normal mice | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | H. Park | - |
dc.contributor.googleauthor | S.-B. Lee | - |
dc.contributor.googleauthor | J. Koh | - |
dc.contributor.googleauthor | J. Kim | - |
dc.identifier.doi | 10.1055/s-0032-1308974 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02845 | - |
dc.contributor.localId | A00921 | - |
dc.contributor.localId | A01747 | - |
dc.relation.journalcode | J00999 | - |
dc.identifier.eissn | 1439-4286 | - |
dc.identifier.pmid | 22499548 | - |
dc.identifier.url | https://www.thieme-connect.de/DOI/DOI?10.1055/s-0032-1308974 | - |
dc.subject.keyword | leptin | - |
dc.subject.keyword | protein stability | - |
dc.subject.keyword | stabilizing peptide | - |
dc.subject.keyword | fusion protein | - |
dc.subject.keyword | animal experiment | - |
dc.subject.keyword | bioefficacy | - |
dc.contributor.alternativeName | Park, Hyun Ju | - |
dc.contributor.alternativeName | Lee, Saet Byul | - |
dc.contributor.alternativeName | Kim, Jong Sun | - |
dc.contributor.affiliatedAuthor | Lee, Saet Byul | - |
dc.contributor.affiliatedAuthor | Kim, Jong Sun | - |
dc.contributor.affiliatedAuthor | Park, Hyun Ju | - |
dc.citation.volume | 44 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 422 | - |
dc.citation.endPage | 428 | - |
dc.identifier.bibliographicCitation | HORMONE AND METABOLIC RESEARCH, Vol.44(6) : 422-428, 2012 | - |
dc.identifier.rimsid | 34174 | - |
dc.type.rims | ART | - |
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