Cited 43 times in
MiR-200b is involved in Tgf-β signaling to regulate mammalian palate development
DC Field | Value | Language |
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dc.contributor.author | 조성원 | - |
dc.contributor.author | 권혁제 | - |
dc.contributor.author | 신정오 | - |
dc.contributor.author | 이민정 | - |
dc.contributor.author | 이종민 | - |
dc.contributor.author | 정한성 | - |
dc.contributor.author | 조경원 | - |
dc.date.accessioned | 2014-12-19T16:52:52Z | - |
dc.date.available | 2014-12-19T16:52:52Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0948-6143 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/90369 | - |
dc.description.abstract | Various cellular and molecular events are involved in palatogenesis, including apoptosis, epithelial-mesenchymal transition (EMT), cell proliferation, and cell migration. Smad2 and Snail, which are well-known key mediators of the transforming growth factor beta (Tgf-β) pathway, play a crucial role in the regulation of palate development. Regulatory effects of microRNA 200b (miR-200b) on Smad2 and Snail in palatogenesis have not yet been elucidated. The aim of this study is to determine the relationship between palate development regulators miR-200b and Tgf-β-mediated genes. Expression of miR-200b, E-cadherin, Smad2, and Snail was detected in the mesenchyme of the mouse palate, while miR-200b was expressed in the medial edge epithelium (MEE) and palatal mesenchyme. After the contact of palatal shelves, miR-200b was no longer expressed in the mesenchyme around the fusion region. The binding activity of miR-200b to both Smad2 and Snail was examined using a luciferase assay. MiR-200b directly targeted Smad2 and Snail at both cellular and molecular levels. The function of miR-200b was determined by overexpression via a lentiviral vector in the palatal shelves. Ectopic expression of miR-200b resulted in suppression of these Tgf-β-mediated regulators and changes of apoptosis and cell proliferation in the palatal fusion region. These results suggest that miR-200b plays a crucial role in regulating the Smad2, Snail, and in apoptosis during palatogenesis by acting as a direct non-coding, influencing factor. Furthermore, the molecular interactions between miR-200b and Tgf-β signaling are important for proper palatogenesis and especially for palate fusion. Elucidating the mechanism of palatogenesis may aid the design of effective gene-based therapies for the treatment of congenital cleft palate. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | HISTOCHEMISTRY AND CELL BIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis | - |
dc.subject.MESH | Cadherins/genetics | - |
dc.subject.MESH | Cadherins/metabolism | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | HEK293 Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunohistochemistry | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred ICR | - |
dc.subject.MESH | MicroRNAs/genetics | - |
dc.subject.MESH | MicroRNAs/metabolism* | - |
dc.subject.MESH | Palate/cytology | - |
dc.subject.MESH | Palate/growth & development* | - |
dc.subject.MESH | Palate/metabolism* | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction | - |
dc.subject.MESH | Signal Transduction*/genetics | - |
dc.subject.MESH | Smad2 Protein/genetics | - |
dc.subject.MESH | Smad2 Protein/metabolism | - |
dc.subject.MESH | Snail Family Transcription Factors | - |
dc.subject.MESH | Transcription Factors/genetics | - |
dc.subject.MESH | Transcription Factors/metabolism | - |
dc.subject.MESH | Transforming Growth Factor beta/genetics | - |
dc.subject.MESH | Transforming Growth Factor beta/metabolism* | - |
dc.title | MiR-200b is involved in Tgf-β signaling to regulate mammalian palate development | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Jeong-Oh Shin | - |
dc.contributor.googleauthor | Jong-Min Lee | - |
dc.contributor.googleauthor | Kyoung-Won Cho | - |
dc.contributor.googleauthor | Sungwook Kwak | - |
dc.contributor.googleauthor | Hyuk-Jae Kwon | - |
dc.contributor.googleauthor | Min-Jung Lee | - |
dc.contributor.googleauthor | Sung-Won Cho | - |
dc.contributor.googleauthor | Kye-Seong Kim | - |
dc.contributor.googleauthor | Han-Sung Jung | - |
dc.identifier.doi | 10.1007/s00418-011-0876-1 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03837 | - |
dc.contributor.localId | A00261 | - |
dc.contributor.localId | A03758 | - |
dc.contributor.localId | A03802 | - |
dc.contributor.localId | A02147 | - |
dc.contributor.localId | A02785 | - |
dc.contributor.localId | A04640 | - |
dc.relation.journalcode | J00992 | - |
dc.identifier.eissn | 1432-119X | - |
dc.identifier.pmid | 22072420 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs00418-011-0876-1 | - |
dc.subject.keyword | Palatogenesis | - |
dc.subject.keyword | MiR-200b | - |
dc.subject.keyword | Smad2 | - |
dc.subject.keyword | Snail | - |
dc.subject.keyword | Apoptosis | - |
dc.subject.keyword | Cell proliferation | - |
dc.contributor.alternativeName | Cho, Sung Won | - |
dc.contributor.alternativeName | Kwon, Hyuk Jae | - |
dc.contributor.alternativeName | Shin, Jeong Oh | - |
dc.contributor.alternativeName | Lee, Min Jung | - |
dc.contributor.alternativeName | Lee, Jong Min | - |
dc.contributor.alternativeName | Jung, Han Sung | - |
dc.contributor.alternativeName | Cho, Kyoung Won | - |
dc.contributor.affiliatedAuthor | Cho, Sung Won | - |
dc.contributor.affiliatedAuthor | Kwon, Hyuk Jae | - |
dc.contributor.affiliatedAuthor | Jung, Han Sung | - |
dc.contributor.affiliatedAuthor | Cho, Kyoung Won | - |
dc.contributor.affiliatedAuthor | Shin, Jeong Oh | - |
dc.contributor.affiliatedAuthor | Lee, Min Jung | - |
dc.contributor.affiliatedAuthor | Lee, Jong Min | - |
dc.citation.volume | 137 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 67 | - |
dc.citation.endPage | 78 | - |
dc.identifier.bibliographicCitation | HISTOCHEMISTRY AND CELL BIOLOGY, Vol.137(1) : 67-78, 2012 | - |
dc.identifier.rimsid | 34168 | - |
dc.type.rims | ART | - |
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