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A novel protein Jpk induces bacterial cell death through reactive oxygen species

DC Field Value Language
dc.contributor.author김명희-
dc.contributor.author공경아-
dc.date.accessioned2014-12-19T16:50:47Z-
dc.date.available2014-12-19T16:50:47Z-
dc.date.issued2012-
dc.identifier.issn0378-1119-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/90304-
dc.description.abstractJpk, a trans-acting regulatory factor associating with the position-specific regulatory element of Hoxa-7, has been reported to induce cell death in both prokaryotic and eukaryotic cells upon overexpression. The N- and C-terminal deleted variants of Jpk were constructed and then the toxicity of each construct was analyzed by checking the viability of the cells and the concomitant morphological changes through electron microscopy following the expression. The N-terminus of Jpk harboring transmembrane domain seemed to be more toxic to bacterial cell than C-terminus and the morphology of bacterial cells expressing N-terminal Jpk was similar to that induced by full length Jpk. The toxicity caused by Jpk protein in bacterial cell was through the production of ROS, which was decreased by an antioxidant (DTT) in a concentration dependent manner. The finding described in this study provides valuable clues on the relationship between Jpk toxicity and ROS generation.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfGENE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBacterial Proteins/physiology*-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCell Death/physiology*-
dc.subject.MESHReactive Oxygen Species/metabolism*-
dc.titleA novel protein Jpk induces bacterial cell death through reactive oxygen species-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Anatomy (해부학)-
dc.contributor.googleauthorSungdo Park-
dc.contributor.googleauthorKyoung-Ah Kong-
dc.contributor.googleauthorMyoung Hee Kim-
dc.identifier.doi10.1016/j.gene.2012.05.044-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00432-
dc.contributor.localIdA00158-
dc.relation.journalcodeJ00921-
dc.identifier.eissn1879-0038-
dc.identifier.pmid22652273-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0378111912006324-
dc.subject.keywordJpk protein-
dc.subject.keywordBacterial toxicity-
dc.subject.keywordROS-
dc.subject.keywordAntioxidant-
dc.subject.keywordHox-
dc.contributor.alternativeNameKim, Myoung Hee-
dc.contributor.alternativeNameKong, Kyoung Ah-
dc.contributor.affiliatedAuthorKim, Myoung Hee-
dc.contributor.affiliatedAuthorKong, Kyoung Ah-
dc.citation.volume504-
dc.citation.number2-
dc.citation.startPage274-
dc.citation.endPage278-
dc.identifier.bibliographicCitationGENE, Vol.504(2) : 274-278, 2012-
dc.identifier.rimsid34130-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers

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