Cited 15 times in
Molecular characterization of drug-resistant and -susceptible Mycobacterium tuberculosis isolated from patients with tuberculosis in Korea
DC Field | Value | Language |
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dc.contributor.author | 조상래 | - |
dc.date.accessioned | 2014-12-19T16:45:13Z | - |
dc.date.available | 2014-12-19T16:45:13Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0732-8893 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/90128 | - |
dc.description.abstract | We investigated the causal relationship between genotype and phenotype of drug-resistant Mycobacterium tuberculosis isolates obtained from patients with pulmonary tuberculosis (TB) in Korea. Of 80 isolates tested, 17, 20, 1, and 7 isolates were mono-resistant to ethambutol (EMB), isoniazid (INH), pyrazinamide (PZA), and rifampicin (RFP), respectively, and 31 isolates (38.8%) were multidrug-resistant (MDR). Sequencing analysis showed that 78% (32/41) of RFP-resistant strains had mutations in the rifampicin resistance-determining region (RRDR) of rpoB, and the mutation at rpoB531 (59.4%) was most abundant. In 52 INH-resistant strains, mutations were found mostly at C-15T (n = 21, 40.4%) in the inhA promoter region as well as at katG315 (n = 12, 23.1%). Mutations at embB306 were mostly found in 26.7% (12/45) of EMB-resistant isolates. New mutations found here in MDR isolates include rpoB523 (Gly523Glu) and embB319 (Tyr319Ser). Consequently, mutations in the rpoB531, C-15T in the inhA promoter region, embB306, and katG315 would be a useful marker for rapid detection of MDR M. tuberculosis isolates in Korea. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Antitubercular Agents/pharmacology* | - |
dc.subject.MESH | Bacterial Proteins/genetics | - |
dc.subject.MESH | Catalase/genetics | - |
dc.subject.MESH | DNA-Directed RNA Polymerases/genetics | - |
dc.subject.MESH | Drug Resistance, Bacterial* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mutation, Missense | - |
dc.subject.MESH | Mycobacterium tuberculosis/drug effects* | - |
dc.subject.MESH | Mycobacterium tuberculosis/isolation & purification* | - |
dc.subject.MESH | Oxidoreductases/genetics | - |
dc.subject.MESH | Point Mutation | - |
dc.subject.MESH | Promoter Regions, Genetic | - |
dc.subject.MESH | Republic of Korea | - |
dc.subject.MESH | Sequence Analysis, DNA | - |
dc.subject.MESH | Tuberculosis, Multidrug-Resistant/microbiology* | - |
dc.subject.MESH | Tuberculosis, Pulmonary/microbiology* | - |
dc.title | Molecular characterization of drug-resistant and -susceptible Mycobacterium tuberculosis isolated from patients with tuberculosis in Korea | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Jee-Hyun Yoon | - |
dc.contributor.googleauthor | Ji-Sun Nam | - |
dc.contributor.googleauthor | Kyung-Jin Kim | - |
dc.contributor.googleauthor | Yeonim Choi | - |
dc.contributor.googleauthor | Hyeyoung Lee | - |
dc.contributor.googleauthor | Sang-Nae Cho | - |
dc.contributor.googleauthor | Young-Tae Ro | - |
dc.identifier.doi | 10.1016/j.diagmicrobio.2011.09.010 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03824 | - |
dc.relation.journalcode | J00731 | - |
dc.identifier.eissn | 1879-0070 | - |
dc.identifier.pmid | 22078903 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0732889311003646 | - |
dc.subject.keyword | Drug resistant | - |
dc.subject.keyword | Korea | - |
dc.subject.keyword | Mutation analysis | - |
dc.subject.keyword | Mycobacterium tuberculosis | - |
dc.subject.keyword | Tuberculosis | - |
dc.contributor.alternativeName | Cho, Sang Nae | - |
dc.contributor.affiliatedAuthor | Cho, Sang Nae | - |
dc.citation.volume | 72 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 52 | - |
dc.citation.endPage | 61 | - |
dc.identifier.bibliographicCitation | DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, Vol.72(1) : 52-61, 2012 | - |
dc.identifier.rimsid | 32377 | - |
dc.type.rims | ART | - |
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