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Endothelial progenitor cell cotransplantation enhances islet engraftment by rapid revascularization.

Authors
 Shinae Kang  ;  Ho Seon Park  ;  Anna Jo  ;  Shin Hee Hong  ;  Han Na Lee  ;  Yeon Yi Lee  ;  Joong Shin Park  ;  Hye Seung Jung  ;  Sung Soo Chung  ;  Kyong Soo Park 
Citation
 DIABETES, Vol.61(4) : 866-876, 2012 
Journal Title
 DIABETES 
ISSN
 0012-1797 
Issue Date
2012
MeSH
Animals ; Blood Glucose ; Cell Proliferation ; Coculture Techniques ; Endothelial Cells/physiology* ; Humans ; Insulin-Secreting Cells/cytology ; Insulin-Secreting Cells/physiology ; Ischemia ; Islets of Langerhans/cytology* ; Islets of Langerhans Transplantation* ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neovascularization, Physiologic/physiology* ; Stem Cell Transplantation*
Keywords
Animals ; Blood Glucose ; Cell Proliferation ; Coculture Techniques ; Endothelial Cells/physiology* ; Humans ; Insulin-Secreting Cells/cytology ; Insulin-Secreting Cells/physiology ; Ischemia ; Islets of Langerhans/cytology* ; Islets of Langerhans Transplantation* ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neovascularization, Physiologic/physiology* ; Stem Cell Transplantation*
Abstract
Impaired revascularization of transplanted islets is a critical problem that leads to progressive islet loss. Since endothelial progenitor cells (EPCs) are known to aid neovascularization, we aimed to enhance islet engraftment by cotransplanting EPCs with islets. Porcine islets, with (islet-EPC group) or without (islet-only group) human cord blood-derived EPCs, were transplanted into diabetic nude mice. The islet-EPC group reached euglycemia by ∼11 days posttransplantation, whereas the islet-only group did not. Also, the islet-EPC group had a higher serum porcine insulin level than the islet-only group. Islets from the islet-EPC group were more rapidly revascularized at the early period of transplantation without increment of final capillary density at the fully revascularized graft. Enhanced revascularization rate in the islet-EPC group was mainly attributed to stimulating vascular endothelial growth factor-A production from the graft. The rapid revascularization by EPC cotransplantation led to better graft perfusion and recovery from hypoxia. EPC cotransplantation was also associated with greater β-cell proliferation, probably by more basement membrane production and hepatocyte growth factor secretion. In conclusion, cotransplantation of EPCs and islets induces better islet engraftment by enhancing the rate of graft revascularization. These findings might provide a directly applicable tool to enhance the efficacy of islet transplantation in clinical practice.
Files in This Item:
T201201826.pdf Download
DOI
22362173
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Shin Ae(강신애) ORCID logo https://orcid.org/0000-0002-9719-4774
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90104
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