3 170

Cited 20 times in

Functional invadopodia formation through stabilization of the PDPN transcript by IMP-3 and cancer-stromal crosstalk for PDPN expression.

DC FieldValueLanguage
dc.contributor.author박광균-
dc.contributor.author장향란-
dc.contributor.author정원윤-
dc.contributor.author황영선-
dc.date.accessioned2014-12-19T16:37:34Z-
dc.date.available2014-12-19T16:37:34Z-
dc.date.issued2012-
dc.identifier.issn0143-3334-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/89883-
dc.description.abstractWe previously reported that insulin-like growth factor-II mRNA-binding protein-3 (IMP-3) depletion (IMP-3(Δ)) was shown to inhibit invadopodia formation and extracellular matrix degradation capacity in oral squamous cell carcinoma (OSCC) cells. In this study, we found that IMP-3(Δ) cells significantly downregulated the podoplanin (PDPN) level, which resulted in a loss of extracellular matrix degradation activity, although invadopodia was still thriving. From RNA in situ hybridization using a digoxigenin-labeled 3'UTR recognition probe of PDPN and reporter assay with 3'UTR of the PDPN gene cloned downstream from the luciferase reporter gene, we revealed that IMP-3 depletion was shown to be downregulated, which most probably lowered PDPN gene expression by reducing mRNA stabilization. In a xenograft model, PDPN depletion was the cause of a decrease in tumor volume and regional infiltration into nearby stroma. Taken together, transforming growth factor beta 1 increased PDPN expression, which potentiated cancer invasion through increased invadopodia formation and extracellular matrix degradation in the low invasive OSCC cell line. Reciprocally, interleukin-1 beta secreted by OSCC cells, stimulated transforming growth factor beta 1 secretion from stromal fibroblasts to induce PDPN expression in OSCC cells. In addition, a retrospective investigation of OSCC patients found that IMP-3 and PDPN expression significantly correlated with lymph node metastasis of OSCC patients. Moreover, co-expression of IMP-3 and PDPN were frequently detected both in primary and lymph nodes metastatic OSCC cells using immunohistochemical dual staining. Thus, the IMP-3-PDPN axis may be a sensitive target molecule in anti-invadopodia therapy for the treatment of metastatic cancers.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfCarcinogenesis-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleFunctional invadopodia formation through stabilization of the PDPN transcript by IMP-3 and cancer-stromal crosstalk for PDPN expression.-
dc.typeArticle-
dc.contributor.collegeResearcher Institutes (부설 연구소)-
dc.contributor.departmentOral Cancer Research Institute (구강종양연구소)-
dc.contributor.googleauthorYoung Sun Hwang-
dc.contributor.googleauthorZhang Xianglan-
dc.contributor.googleauthorKwang-Kyun Park-
dc.contributor.googleauthorWon-Yoon Chung-
dc.identifier.doi10.1093/carcin/bgs258-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01429-
dc.contributor.localIdA03489-
dc.contributor.localIdA03676-
dc.contributor.localIdA04472-
dc.relation.journalcodeJ00456-
dc.identifier.urlhttp://carcin.oxfordjournals.org/content/33/11/2135.long-
dc.contributor.alternativeNamePark, Kwang Kyun-
dc.contributor.alternativeNameZhang, Xiang Lan-
dc.contributor.alternativeNameChung, Won Yoon-
dc.contributor.alternativeNameHwang, Young Sun-
dc.contributor.affiliatedAuthorPark, Kwang Kyun-
dc.contributor.affiliatedAuthorZhang, Xiang Lan-
dc.contributor.affiliatedAuthorChung, Won Yoon-
dc.contributor.affiliatedAuthorHwang, Young Sun-
dc.citation.volume33-
dc.citation.number11-
dc.citation.startPage2135-
dc.citation.endPage2146-
dc.identifier.bibliographicCitationCarcinogenesis, Vol.33(11) : 2135-2146, 2012-
Appears in Collections:
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.