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Breast cancers presenting luminal B subtype features show higher discordant human epidermal growth factor receptor 2 results between immunohistochemistry and fluorescence in situ hybridization.

Authors
 Seho Park  ;  Hyung Seok Park  ;  Ja Seung Koo,  ;  Woo Ick Yang  ;  Seung Il Kim  ;  Byeong-Woo Park 
Citation
 CANCER, Vol.118(4) : 914-923, 2012 
Journal Title
 CANCER 
ISSN
 0008-543X 
Issue Date
2012
MeSH
Adult ; Biomarkers, Tumor/metabolism* ; Breast Neoplasms/metabolism* ; Breast Neoplasms/mortality ; Breast Neoplasms/pathology* ; Cell Differentiation ; Cell Proliferation ; Chi-Square Distribution ; Female ; Humans ; Immunohistochemistry/methods* ; In Situ Hybridization, Fluorescence/methods* ; Kaplan-Meier Estimate ; Middle Aged ; Prognosis ; Proportional Hazards Models ; Receptor, ErbB-2/metabolism* ; Receptors, Estrogen/metabolism ; Retrospective Studies ; Survival Rate
Keywords
Adult ; Biomarkers, Tumor/metabolism* ; Breast Neoplasms/metabolism* ; Breast Neoplasms/mortality ; Breast Neoplasms/pathology* ; Cell Differentiation ; Cell Proliferation ; Chi-Square Distribution ; Female ; Humans ; Immunohistochemistry/methods* ; In Situ Hybridization, Fluorescence/methods* ; Kaplan-Meier Estimate ; Middle Aged ; Prognosis ; Proportional Hazards Models ; Receptor, ErbB-2/metabolism* ; Receptors, Estrogen/metabolism ; Retrospective Studies ; Survival Rate
Abstract
BACKGROUND: The aims of this study were to compare human epidermal growth factor receptor 2 (HER2) results between immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), and to investigate the clinicopathological characteristics and outcomes according to their results. METHODS: Using consecutive tissue microarrays, IHC and FISH were performed as guidelines in 950 invasive breast cancers treated between November 1999 and August 2005. Characteristics and outcomes were retrospectively analyzed using a chi-square test, the Kaplan-Meier method, and Cox's model. RESULTS: FISH-positivity was observed in 2.6%, 4.8%, 28.1%, and 93.8% of IHC 0, 1+, 2+, and 3+, respectively, and the concordance rate between the 2 assays was 95.5%. IHC-positive or FISH-positive cases were associated with poorer differentiation, negative expression of hormone receptors, and higher proliferative index. Among IHC-equivocal or IHC-negative patients, positive FISH was negatively associated with survival in univariate and multivariate analyses. Among IHC-negative patients, tumors showing luminal B subtype features such as estrogen receptor (ER)-positive, grade II/III, and high Ki-67 presented discordantly high FISH-positivity. Among IHC-positive cases, FISH was not related to outcomes. CONCLUSIONS: The result of FISH is significantly related to prognosis of patients with IHC-negative or IHC-equivocal result. Therefore, FISH should be performed in IHC-equivocal cases. FISH assay might be considered for a selected group of patients with IHC-negative tumors showing luminal B subtype features of ER-positive, grade II/III, and high Ki-67 expression.
Files in This Item:
T201200620.pdf Download
DOI
21800290
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Koo, Ja Seung(구자승) ORCID logo https://orcid.org/0000-0003-4546-4709
Kim, Seung Il(김승일)
Park, Byeong Woo(박병우) ORCID logo https://orcid.org/0000-0003-1353-2607
Park, Se Ho(박세호) ORCID logo https://orcid.org/0000-0001-8089-2755
Park, Hyung Seok(박형석) ORCID logo https://orcid.org/0000-0001-5322-6036
Yang, Woo Ick(양우익) ORCID logo https://orcid.org/0000-0002-6084-5019
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/89851
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