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Dual optical biosensors for imaging microRNA-1 during myogenesis

DC Field Value Language
dc.contributor.author강원준-
dc.contributor.author이종두-
dc.contributor.author조예림-
dc.contributor.author채주리-
dc.date.accessioned2014-12-19T16:33:00Z-
dc.date.available2014-12-19T16:33:00Z-
dc.date.issued2012-
dc.identifier.issn0142-9612-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/89740-
dc.description.abstractDual optical microRNA (miRNA) imaging systems, bioluminescent reporter gene (a signal-off mechanism)- or fluorescent molecular beacon (MB) (a signal-on mechanism)-based miRNA imaging system, have individually allowed us to sense miRNA biogenesis in a noninvasive and iterative manner. Both of these imaging systems have shortcomings with respect to image quality. Therefore, we designed a dual optical imaging systems by simultaneous imaging of a miRNA-1 reporter gene (CMV/Gluc/3xPT_miR-1) and miRNA-1 MB in a single cell to overcome these limitations and used it to visualize miRNA-1, a highly expressed miRNA in cardiac and skeletal muscle. During myogenic differentiation of C2C12 cells, the bioluminescence intensity from CMV/Gluc/3xPT_miR-1 revealed a miRNA-1-dependent gradual decrease and the fluorescence intensity from miR-1 MB demonstrated a miRNA-1-dependent gradual increase both in vitro and in vivo. The dual miRNA-1 imaging systems, which provides the complementary imaging information about miRNA biogenesis, could be useful to sense miRNA expression during various biologic processes.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfBIOMATERIALS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBiosensing Techniques/methods*-
dc.subject.MESHDiagnostic Imaging/methods*-
dc.subject.MESHFluorescence-
dc.subject.MESHGene Expression Regulation-
dc.subject.MESHGenes, Reporter/genetics-
dc.subject.MESHHeLa Cells-
dc.subject.MESHHumans-
dc.subject.MESHLuminescent Measurements-
dc.subject.MESHMice-
dc.subject.MESHMicroRNAs/genetics-
dc.subject.MESHMicroRNAs/metabolism*-
dc.subject.MESHMolecular Probes/metabolism-
dc.subject.MESHMuscle Development*-
dc.subject.MESHOptical Devices*-
dc.subject.MESHReproducibility of Results-
dc.titleDual optical biosensors for imaging microRNA-1 during myogenesis-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.googleauthorWon Jun Kang-
dc.contributor.googleauthorYe Lim Cho-
dc.contributor.googleauthorJu Ri Chae-
dc.contributor.googleauthorJong Doo Lee-
dc.contributor.googleauthorBahy A. Ali-
dc.contributor.googleauthorAbdulaziz A. Al-Khedhairy-
dc.contributor.googleauthorChang Hyun Lee-
dc.contributor.googleauthorSoonhag Kim-
dc.identifier.doi10.1016/j.biomaterials.2012.05.056-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00062-
dc.contributor.localIdA03138-
dc.contributor.localIdA03862-
dc.contributor.localIdA04023-
dc.relation.journalcodeJ00312-
dc.identifier.eissn1878-5905-
dc.identifier.pmid22698721-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0142961212005972-
dc.subject.keywordBiosensor-
dc.subject.keywordmicroRNA-1-
dc.subject.keywordMolecular beacon-
dc.subject.keywordReporter gene-
dc.subject.keywordMyogenic differentiation-
dc.subject.keywordMolecular imaging-
dc.contributor.alternativeNameKang, Won Jun-
dc.contributor.alternativeNameLee, Jong Doo-
dc.contributor.alternativeNameCho, Ye Lim-
dc.contributor.alternativeNameChae, Ju Ri-
dc.contributor.affiliatedAuthorKang, Won Jun-
dc.contributor.affiliatedAuthorLee, Jong Doo-
dc.contributor.affiliatedAuthorCho, Ye Lim-
dc.contributor.affiliatedAuthorChae, Ju Ri-
dc.citation.volume33-
dc.citation.number27-
dc.citation.startPage6430-
dc.citation.endPage6437-
dc.identifier.bibliographicCitationBIOMATERIALS, Vol.33(27) : 6430-6437, 2012-
dc.identifier.rimsid31876-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Nuclear Medicine (핵의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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