Cited 0 times in
Dual optical biosensors for imaging microRNA-1 during myogenesis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 강원준 | - |
dc.contributor.author | 이종두 | - |
dc.contributor.author | 조예림 | - |
dc.contributor.author | 채주리 | - |
dc.date.accessioned | 2014-12-19T16:33:00Z | - |
dc.date.available | 2014-12-19T16:33:00Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/89740 | - |
dc.description.abstract | Dual optical microRNA (miRNA) imaging systems, bioluminescent reporter gene (a signal-off mechanism)- or fluorescent molecular beacon (MB) (a signal-on mechanism)-based miRNA imaging system, have individually allowed us to sense miRNA biogenesis in a noninvasive and iterative manner. Both of these imaging systems have shortcomings with respect to image quality. Therefore, we designed a dual optical imaging systems by simultaneous imaging of a miRNA-1 reporter gene (CMV/Gluc/3xPT_miR-1) and miRNA-1 MB in a single cell to overcome these limitations and used it to visualize miRNA-1, a highly expressed miRNA in cardiac and skeletal muscle. During myogenic differentiation of C2C12 cells, the bioluminescence intensity from CMV/Gluc/3xPT_miR-1 revealed a miRNA-1-dependent gradual decrease and the fluorescence intensity from miR-1 MB demonstrated a miRNA-1-dependent gradual increase both in vitro and in vivo. The dual miRNA-1 imaging systems, which provides the complementary imaging information about miRNA biogenesis, could be useful to sense miRNA expression during various biologic processes. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Biosensing Techniques/methods* | - |
dc.subject.MESH | Diagnostic Imaging/methods* | - |
dc.subject.MESH | Fluorescence | - |
dc.subject.MESH | Gene Expression Regulation | - |
dc.subject.MESH | Genes, Reporter/genetics | - |
dc.subject.MESH | HeLa Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Luminescent Measurements | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | MicroRNAs/genetics | - |
dc.subject.MESH | MicroRNAs/metabolism* | - |
dc.subject.MESH | Molecular Probes/metabolism | - |
dc.subject.MESH | Muscle Development* | - |
dc.subject.MESH | Optical Devices* | - |
dc.subject.MESH | Reproducibility of Results | - |
dc.title | Dual optical biosensors for imaging microRNA-1 during myogenesis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.googleauthor | Won Jun Kang | - |
dc.contributor.googleauthor | Ye Lim Cho | - |
dc.contributor.googleauthor | Ju Ri Chae | - |
dc.contributor.googleauthor | Jong Doo Lee | - |
dc.contributor.googleauthor | Bahy A. Ali | - |
dc.contributor.googleauthor | Abdulaziz A. Al-Khedhairy | - |
dc.contributor.googleauthor | Chang Hyun Lee | - |
dc.contributor.googleauthor | Soonhag Kim | - |
dc.identifier.doi | 10.1016/j.biomaterials.2012.05.056 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00062 | - |
dc.contributor.localId | A03138 | - |
dc.contributor.localId | A03862 | - |
dc.contributor.localId | A04023 | - |
dc.relation.journalcode | J00312 | - |
dc.identifier.eissn | 1878-5905 | - |
dc.identifier.pmid | 22698721 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0142961212005972 | - |
dc.subject.keyword | Biosensor | - |
dc.subject.keyword | microRNA-1 | - |
dc.subject.keyword | Molecular beacon | - |
dc.subject.keyword | Reporter gene | - |
dc.subject.keyword | Myogenic differentiation | - |
dc.subject.keyword | Molecular imaging | - |
dc.contributor.alternativeName | Kang, Won Jun | - |
dc.contributor.alternativeName | Lee, Jong Doo | - |
dc.contributor.alternativeName | Cho, Ye Lim | - |
dc.contributor.alternativeName | Chae, Ju Ri | - |
dc.contributor.affiliatedAuthor | Kang, Won Jun | - |
dc.contributor.affiliatedAuthor | Lee, Jong Doo | - |
dc.contributor.affiliatedAuthor | Cho, Ye Lim | - |
dc.contributor.affiliatedAuthor | Chae, Ju Ri | - |
dc.citation.volume | 33 | - |
dc.citation.number | 27 | - |
dc.citation.startPage | 6430 | - |
dc.citation.endPage | 6437 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, Vol.33(27) : 6430-6437, 2012 | - |
dc.identifier.rimsid | 31876 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.