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The effects of sub-lethal concentrations of silver nanoparticles on inflammatory and stress genes in human macrophages using cDNA microarray analysis
DC Field | Value | Language |
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dc.contributor.author | 최인홍 | - |
dc.date.accessioned | 2014-12-19T16:32:47Z | - |
dc.date.available | 2014-12-19T16:32:47Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/89733 | - |
dc.description.abstract | Because of the limited information on size-dependent particle-mediated effects, the present study was conducted to determine if the changes in induced protein expression between 5 nm silver nanoparticles and 100 nm particles after exposure to sub-lethal concentrations. A total of 28,000 cDNA profiles were screened using 5 nm silver nanoparticles and 100 nm silver nanoparticles in a macrophage cell line. Based on results obtained from cDNA microarray we also assessed protein levels of hemeoxygenase-1 (HO-1), heat shock protein-70 (HSP-70) and interleukin-8 (IL-8), which were shown to significantly increase. Together with results obtained using N-acetylcystein (NAC), we were able to clearly show that low level and early stage exposure to 5 nm silver nanoparticles, but not 100 nm, induces expression of IL-8 as well as stress genes against reactive oxygen species (ROS). Therefore, we provide important data to understand and identify the early effects of silver nanoparticles on the immune system. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 4690~4699 | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cell Survival/drug effects | - |
dc.subject.MESH | HSP70 Heat-Shock Proteins/metabolism | - |
dc.subject.MESH | Heme Oxygenase-1/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interleukin-8/metabolism | - |
dc.subject.MESH | Macrophages/drug effects* | - |
dc.subject.MESH | Macrophages/metabolism* | - |
dc.subject.MESH | Metal Nanoparticles/adverse effects | - |
dc.subject.MESH | Metal Nanoparticles/chemistry | - |
dc.subject.MESH | Metal Nanoparticles/therapeutic use* | - |
dc.subject.MESH | Metal Nanoparticles/ultrastructure | - |
dc.subject.MESH | Microscopy, Electron, Transmission | - |
dc.subject.MESH | Oligonucleotide Array Sequence Analysis/methods* | - |
dc.subject.MESH | Reactive Oxygen Species/metabolism | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction | - |
dc.subject.MESH | Silver/adverse effects | - |
dc.subject.MESH | Silver/chemistry | - |
dc.subject.MESH | Silver/therapeutic use* | - |
dc.title | The effects of sub-lethal concentrations of silver nanoparticles on inflammatory and stress genes in human macrophages using cDNA microarray analysis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Dae-Hyoun Lim | - |
dc.contributor.googleauthor | Jiyoung Jang | - |
dc.contributor.googleauthor | Seungjae Kim | - |
dc.contributor.googleauthor | Taegyeong Kang | - |
dc.contributor.googleauthor | Kangtaek Lee | - |
dc.contributor.googleauthor | In-Hong Choi | - |
dc.identifier.doi | 22459196 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04167 | - |
dc.relation.journalcode | J00312 | - |
dc.identifier.eissn | 1878-5905 | - |
dc.identifier.pmid | 22459196 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0142961212002785 | - |
dc.subject.keyword | Nanoparticle | - |
dc.subject.keyword | Silver | - |
dc.subject.keyword | Macrophage | - |
dc.subject.keyword | Interleukin | - |
dc.subject.keyword | mRNA | - |
dc.contributor.alternativeName | Choi, In Hong | - |
dc.contributor.affiliatedAuthor | Choi, In Hong | - |
dc.citation.volume | 33 | - |
dc.citation.number | 18 | - |
dc.citation.startPage | 4690 | - |
dc.citation.endPage | 4699 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, Vol.33(18) : 4690-4699, 2012 | - |
dc.identifier.rimsid | 31874 | - |
dc.type.rims | ART | - |
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