Cited 31 times in
The MCP-1/CCR2 axis in podocytes is involved in apoptosis induced by diabetic conditions
DC Field | Value | Language |
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dc.contributor.author | 강신욱 | - |
dc.contributor.author | 강혜영 | - |
dc.contributor.author | 한승혁 | - |
dc.contributor.author | 곽승재 | - |
dc.contributor.author | 김승혜 | - |
dc.contributor.author | 남보영 | - |
dc.contributor.author | 박정탁 | - |
dc.contributor.author | 유태현 | - |
dc.contributor.author | 이순하 | - |
dc.contributor.author | 팽지선 | - |
dc.date.accessioned | 2014-12-19T16:28:18Z | - |
dc.date.available | 2014-12-19T16:28:18Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1360-8185 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/89590 | - |
dc.description.abstract | Previous studies have demonstrated the importance of monocyte chemoattractant protein-1 (MCP-1) in the pathogenesis of diabetic nephropathy in terms of inflammation, but the direct role of the MCP-1/CCR2 system on podocyte apoptosis under diabetic conditions has never been explored. In vitro, mouse podocytes were exposed to a medium containing 30 mM glucose (HG) with or without CCR2 siRNA or CCR2 inhibitor (RS102895). Podocytes were also treated with MCP-1 or TGF-β1 with or without anti-TGF-β1 antibody, CCR2 siRNA, or CCR2 inhibitor. In vivo, 20 db/m and 20 db/db mice were divided into two groups, and ten mice from each group were treated with RS102895. Western blot and Hoechst 33342 or TUNEL staining were performed to identify apoptosis. HG-induced apoptosis and TGF-β1 levels were significantly abrogated by CCR2 inhibition. In addition, treatment with MCP-1 directly induced apoptosis via CCR2. Moreover, TGF-β1- and MCP-1-induced apoptosis were significantly ameliorated by the inhibition of CCR2 and anti-TGF-β1 antibody, respectively. Glomerular expression of cleaved caspase-3 and apoptotic cells within glomeruli were also significantly increased in db/db mice compared to db/m mice, and these increases were significantly attenuated in db/db + RS102895 mice. These results suggest that interactions between the MCP-1/CCR2 system and TGF-β1 may contribute to podocyte apoptosis under diabetic conditions. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | APOPTOSIS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis* | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Chemokine CCL2/genetics | - |
dc.subject.MESH | Chemokine CCL2/metabolism* | - |
dc.subject.MESH | Diabetic Nephropathies/genetics | - |
dc.subject.MESH | Diabetic Nephropathies/metabolism | - |
dc.subject.MESH | Diabetic Nephropathies/physiopathology* | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Glucose | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Podocytes/cytology* | - |
dc.subject.MESH | Podocytes/metabolism | - |
dc.subject.MESH | Receptors, CCR2/genetics | - |
dc.subject.MESH | Receptors, CCR2/metabolism* | - |
dc.subject.MESH | Transforming Growth Factor beta1/metabolism | - |
dc.title | The MCP-1/CCR2 axis in podocytes is involved in apoptosis induced by diabetic conditions | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Bo Young Nam | - |
dc.contributor.googleauthor | Jisun Paeng | - |
dc.contributor.googleauthor | Seung Hye Kim | - |
dc.contributor.googleauthor | Sun Ha Lee | - |
dc.contributor.googleauthor | Do Hee Kim | - |
dc.contributor.googleauthor | Hye-Young Kang | - |
dc.contributor.googleauthor | Jin Ji Li | - |
dc.contributor.googleauthor | Seung-Jae Kwak | - |
dc.contributor.googleauthor | Jung Tak Park | - |
dc.contributor.googleauthor | Tae-Hyun Yoo | - |
dc.contributor.googleauthor | Seung Hyeok Han | - |
dc.contributor.googleauthor | Dong Ki Kim | - |
dc.contributor.googleauthor | Shin-Wook Kang | - |
dc.identifier.doi | 10.1007/s10495-011-0661-6 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00053 | - |
dc.contributor.localId | A00096 | - |
dc.contributor.localId | A04304 | - |
dc.contributor.localId | A00170 | - |
dc.contributor.localId | A01251 | - |
dc.contributor.localId | A01654 | - |
dc.contributor.localId | A02526 | - |
dc.contributor.localId | A02908 | - |
dc.contributor.localId | A04241 | - |
dc.contributor.localId | A00665 | - |
dc.relation.journalcode | J00195 | - |
dc.identifier.eissn | 1573-675X | - |
dc.identifier.pmid | 22006533 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs10495-011-0661-6 | - |
dc.subject.keyword | Diabetic nephropathy | - |
dc.subject.keyword | Podocytes | - |
dc.subject.keyword | Monocyte chemoattractant protein-1 | - |
dc.subject.keyword | Transforming growth factor-β1 | - |
dc.subject.keyword | Apoptosis | - |
dc.contributor.alternativeName | Kang, Shin Wook | - |
dc.contributor.alternativeName | Kang, Hye Young | - |
dc.contributor.alternativeName | Han, Seung Hyeok | - |
dc.contributor.alternativeName | Kwak, Seung Jae | - |
dc.contributor.alternativeName | Kim, Seung Hye | - |
dc.contributor.alternativeName | Nam, Bo Young | - |
dc.contributor.alternativeName | Park, Jung Tak | - |
dc.contributor.alternativeName | Yoo, Tae Hyun | - |
dc.contributor.alternativeName | Lee, Sun Ha | - |
dc.contributor.alternativeName | Paeng, Ji Sun | - |
dc.contributor.affiliatedAuthor | Kang, Shin Wook | - |
dc.contributor.affiliatedAuthor | Kang, Hye Young | - |
dc.contributor.affiliatedAuthor | Han, Seung Hyeok | - |
dc.contributor.affiliatedAuthor | Kwak, Seung Jae | - |
dc.contributor.affiliatedAuthor | Nam, Bo Young | - |
dc.contributor.affiliatedAuthor | Park, Jung Tak | - |
dc.contributor.affiliatedAuthor | Yoo, Tae Hyun | - |
dc.contributor.affiliatedAuthor | Lee, Sun Ha | - |
dc.contributor.affiliatedAuthor | Paeng, Ji Sun | - |
dc.contributor.affiliatedAuthor | Kim, Seung Hye | - |
dc.citation.volume | 17 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 13 | - |
dc.identifier.bibliographicCitation | APOPTOSIS, Vol.17(1) : 1-13, 2012 | - |
dc.identifier.rimsid | 32335 | - |
dc.type.rims | ART | - |
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