Cited 19 times in
Dual oxidase 2 is essential for the toll-like receptor 5-mediated inflammatory response in airway mucosa
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김창훈 | - |
dc.contributor.author | 유지환 | - |
dc.contributor.author | 윤주헌 | - |
dc.contributor.author | 이상남 | - |
dc.contributor.author | 주정희 | - |
dc.date.accessioned | 2014-12-19T16:27:59Z | - |
dc.date.available | 2014-12-19T16:27:59Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1523-0864 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/89580 | - |
dc.description.abstract | AIMS: Airway mucosa is constantly exposed to various airborne microbes, and epithelial host defense requires a robust innate immunity. Recently, it has been suggested that NADPH oxidase (NOX) isozymes serve functional roles in toll-like receptor (TLR)-mediated innate immune responses. However, the molecular mechanism between TLR and NOX-mediated reactive oxygen species (ROS) production in human airway mucosa has been poorly understood. RESULTS: Here, we show that flagellin-induced ROS generation is dependent on dual oxidase 2 (DUOX2) activation, which is regulated by [Ca(2+)](i) mobilization in primary normal human nasal epithelial (NHNE) cells. Interestingly, we observed that silencing of DUOX2 expression in NHNE cells and nasal epithelium of Duox2 knockout mice failed to trigger mucin and MIP-2? production upon challenging flagellin. INNOVATION: Our observation in this study reveals that flagellin-induced hydrogen peroxide (H(2)O(2)) generation is critical for TLR5-dependent innate immune responses, including IL-8 production and MUC5AC expression in the nasal epithelium. Furthermore, DUOX2-mediated H(2)O(2) generation activated by the flagellin-TLR5 axis might serve as a novel therapeutic target for infectious inflammation diseases in the airway tract. CONCLUSION: Taken together, we propose that DUOX2 plays pivotal roles in TLR5-dependent inflammatory response of nasal airway epithelium. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | ANTIOXIDANTS & REDOX SIGNALING | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Calcium/metabolism | - |
dc.subject.MESH | Cluster Analysis | - |
dc.subject.MESH | Cytosol/metabolism | - |
dc.subject.MESH | Dual Oxidases | - |
dc.subject.MESH | Epithelial Cells/metabolism | - |
dc.subject.MESH | Flagellin/metabolism | - |
dc.subject.MESH | Gene Expression Profiling | - |
dc.subject.MESH | Gene Expression Regulation | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Inflammation/immunology | - |
dc.subject.MESH | Inflammation/metabolism* | - |
dc.subject.MESH | Interleukin-8/immunology | - |
dc.subject.MESH | Interleukin-8/metabolism | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, 129 Strain | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Mice, Knockout | - |
dc.subject.MESH | Mucin 5AC/immunology | - |
dc.subject.MESH | Mucin 5AC/metabolism | - |
dc.subject.MESH | Mutation | - |
dc.subject.MESH | NADPH Oxidases/genetics | - |
dc.subject.MESH | NADPH Oxidases/metabolism* | - |
dc.subject.MESH | Nasal Lavage Fluid/immunology | - |
dc.subject.MESH | Neutrophil Infiltration/immunology | - |
dc.subject.MESH | Reactive Oxygen Species/metabolism | - |
dc.subject.MESH | Respiratory Mucosa/immunology | - |
dc.subject.MESH | Respiratory Mucosa/metabolism* | - |
dc.subject.MESH | Toll-Like Receptor 5/immunology | - |
dc.subject.MESH | Toll-Like Receptor 5/metabolism* | - |
dc.title | Dual oxidase 2 is essential for the toll-like receptor 5-mediated inflammatory response in airway mucosa | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | 생체방어연구센터 | - |
dc.contributor.googleauthor | Jung-Hee Joo | - |
dc.contributor.googleauthor | Ji-Hwan Ryu | - |
dc.contributor.googleauthor | Chang-Hoon Kim | - |
dc.contributor.googleauthor | Hyun Jik Kim | - |
dc.contributor.googleauthor | Mi-Sun Suh | - |
dc.contributor.googleauthor | Jin-Oh Kim | - |
dc.contributor.googleauthor | Seung Yeun Chung | - |
dc.contributor.googleauthor | Sang Nam Lee | - |
dc.contributor.googleauthor | Hwan Mook Kim | - |
dc.contributor.googleauthor | Yun Soo Bae | - |
dc.contributor.googleauthor | Joo-Heon Yoon | - |
dc.identifier.doi | 10.1196/annals.1378.032 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02604 | - |
dc.contributor.localId | A02813 | - |
dc.contributor.localId | A03958 | - |
dc.contributor.localId | A01050 | - |
dc.contributor.localId | A02522 | - |
dc.relation.journalcode | J00190 | - |
dc.identifier.eissn | 1557-7716 | - |
dc.identifier.pmid | 21714724 | - |
dc.identifier.url | http://online.liebertpub.com/doi/abs/10.1089/ars.2011.3898 | - |
dc.subject.keyword | Animals | - |
dc.subject.keyword | Calcium/metabolism | - |
dc.subject.keyword | Cluster Analysis | - |
dc.subject.keyword | Cytosol/metabolism | - |
dc.subject.keyword | Dual Oxidases | - |
dc.subject.keyword | Epithelial Cells/metabolism | - |
dc.subject.keyword | Flagellin/metabolism | - |
dc.subject.keyword | Gene Expression Profiling | - |
dc.subject.keyword | Gene Expression Regulation | - |
dc.subject.keyword | Humans | - |
dc.subject.keyword | Inflammation/immunology | - |
dc.subject.keyword | Inflammation/metabolism* | - |
dc.subject.keyword | Interleukin-8/immunology | - |
dc.subject.keyword | Interleukin-8/metabolism | - |
dc.subject.keyword | Mice | - |
dc.subject.keyword | Mice, 129 Strain | - |
dc.subject.keyword | Mice, Inbred C57BL | - |
dc.subject.keyword | Mice, Knockout | - |
dc.subject.keyword | Mucin 5AC/immunology | - |
dc.subject.keyword | Mucin 5AC/metabolism | - |
dc.subject.keyword | Mutation | - |
dc.subject.keyword | NADPH Oxidases/genetics | - |
dc.subject.keyword | NADPH Oxidases/metabolism* | - |
dc.subject.keyword | Nasal Lavage Fluid/immunology | - |
dc.subject.keyword | Neutrophil Infiltration/immunology | - |
dc.subject.keyword | Reactive Oxygen Species/metabolism | - |
dc.subject.keyword | Respiratory Mucosa/immunology | - |
dc.subject.keyword | Respiratory Mucosa/metabolism* | - |
dc.subject.keyword | Toll-Like Receptor 5/immunology | - |
dc.subject.keyword | Toll-Like Receptor 5/metabolism* | - |
dc.contributor.alternativeName | Kim, Chang Hoon | - |
dc.contributor.alternativeName | Ryu, Ji Hwan | - |
dc.contributor.alternativeName | Yoon, Joo Heon | - |
dc.contributor.alternativeName | Lee, Sang Nam | - |
dc.contributor.alternativeName | Joo, Jung Hee | - |
dc.contributor.affiliatedAuthor | Yoon, Joo Heon | - |
dc.contributor.affiliatedAuthor | Lee, Sang Nam | - |
dc.contributor.affiliatedAuthor | Joo, Jung Hee | - |
dc.contributor.affiliatedAuthor | Kim, Chang Hoon | - |
dc.contributor.affiliatedAuthor | Ryu, Ji Hwan | - |
dc.citation.volume | 16 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 57 | - |
dc.citation.endPage | 70 | - |
dc.identifier.bibliographicCitation | ANTIOXIDANTS & REDOX SIGNALING, Vol.16(1) : 57-70, 2012 | - |
dc.identifier.rimsid | 32328 | - |
dc.type.rims | ART | - |
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