3 174

Cited 0 times in

Isoflurane-induced post-conditioning in senescent hearts is attenuated by failure to activate reperfusion injury salvage kinase pathway

Authors
 D. J. CHANG  ;  C. H. CHANG  ;  J. S. KIM  ;  Y. W. HONG  ;  W. K. LEE  ;  Y. H. SHIM 
Citation
 ACTA ANAESTHESIOLOGICA SCANDINAVICA, Vol.56(7) : 896-903, 2012 
Journal Title
 ACTA ANAESTHESIOLOGICA SCANDINAVICA 
ISSN
 0001-5172 
Issue Date
2012
MeSH
Aging/physiology* ; Animals ; Cardiotonic Agents/pharmacology ; Cardiotonic Agents/therapeutic use* ; Drug Evaluation, Preclinical ; Glycogen Synthase Kinase 3/physiology* ; Glycogen Synthase Kinase 3 beta ; Ischemic Postconditioning/methods* ; Isoflurane/pharmacology ; Isoflurane/therapeutic use* ; MAP Kinase Signaling System* ; Male ; Mitogen-Activated Protein Kinase 1/physiology* ; Mitogen-Activated Protein Kinase 3/physiology* ; Myocardial Infarction/pathology ; Myocardial Reperfusion ; Myocardial Reperfusion Injury/enzymology ; Myocardial Reperfusion Injury/physiopathology ; Myocardial Reperfusion Injury/prevention & control* ; Phosphorylation ; Protein Processing, Post-Translational ; Proto-Oncogene Proteins c-akt/physiology* ; Random Allocation ; Rats ; Rats, Wistar
Keywords
Aging/physiology* ; Animals ; Cardiotonic Agents/pharmacology ; Cardiotonic Agents/therapeutic use* ; Drug Evaluation, Preclinical ; Glycogen Synthase Kinase 3/physiology* ; Glycogen Synthase Kinase 3 beta ; Ischemic Postconditioning/methods* ; Isoflurane/pharmacology ; Isoflurane/therapeutic use* ; MAP Kinase Signaling System* ; Male ; Mitogen-Activated Protein Kinase 1/physiology* ; Mitogen-Activated Protein Kinase 3/physiology* ; Myocardial Infarction/pathology ; Myocardial Reperfusion ; Myocardial Reperfusion Injury/enzymology ; Myocardial Reperfusion Injury/physiopathology ; Myocardial Reperfusion Injury/prevention & control* ; Phosphorylation ; Protein Processing, Post-Translational ; Proto-Oncogene Proteins c-akt/physiology* ; Random Allocation ; Rats ; Rats, Wistar
Abstract
BACKGROUND: We investigated the cardioprotective effects of isoflurane administered at the onset of reperfusion in senescent rat in vivo, and the activation of the reperfusion injury salvage kinase (RISK) pathway to address a possible mechanism underlying age-related differences. METHODS: Male Wistar rats were assigned to age groups (young, 3-5 months; old, 20-24 months), and randomly selected to receive isoflurane (1 minimum alveolar concentration) or not for 3 min before and 2 min after reperfusion (ISO postC). Rats were subjected to coronary occlusion for 30 min followed by 2 h of reperfusion. Western blot analysis was used to assess the phosphorylation of extracellular signal-regulated kinase (ERK1/2), Akt, and GSK3β 15 min after reperfusion. RESULTS: Brief administration of isoflurane 3 min before and 2 min after the initiation of early reperfusion reduced infarct size (56 ± 8% of left ventricular area at risk, mean ± standard deviation) compared with controls (68 ± 4%) in young rats, but had no effect in old rats (56 ± 8% in ISO postC and 56 ± 10% in control, respectively). Phosphorylation of ERK1/2, Akt, and GSK3β were increased in the young ISO postC group but not in the old ISO postC group compared with control groups of the respective ages. CONCLUSIONS: We demonstrated that isoflurane post-conditions the heart in young but not in senescent rats. Failure to activate RISK pathway may contribute to attenuation of isoflurane-induced post-conditioning effect in senescent rats.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1399-6576.2012.02702.x/abstract
DOI
22571393
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
Yonsei Authors
Shim, Yon Hee(심연희) ORCID logo https://orcid.org/0000-0003-1921-3391
Chang, Chul Ho(장철호) ORCID logo https://orcid.org/0000-0001-5647-8298
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/89351
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse