3 257

Cited 19 times in

A Trachea-Inspired Bifurcated Microfilter Capturing Viable Circulating Tumor Cells via Altered Biophysical Properties as Measured by Atomic Force Microscopy

 Minseok S. Kim  ;  Jinhoon Kim  ;  Wonho Lee  ;  Sang-Joon Cho  ;  Jin-Mi Oh  ;  June-Young Lee  ;  Sanghyun Baek  ;  Yeon Jeong Kim  ;  Tae Seok Sim  ;  Hun Joo Lee  ;  Goo-Eun Jung  ;  Seung-Il Kim  ;  Jong-Myeon Park  ;  Jin Ho Oh  ;  Ogan Gurel  ;  Soo Suk Lee  ;  Jeong-Gun Lee 
 SMALL, Vol.9(18) : 3103-3110, 2013 
Journal Title
Issue Date
Humans ; Microfluidic Analytical Techniques/methods* ; Microfluidics ; Microscopy, Atomic Force/methods* ; Neoplastic Cells, Circulating/metabolism* ; Trachea*
biophysics ; cancer diagnostics ; circulating tumor cells ; filters ; microfluidics
Circulating tumor cells (CTCs), though exceedingly rare in the blood, are nonetheless becoming increasingly important in cancer diagnostics. Despite this keen interest and the growing number of potential clinical applications, there has been limited success in developing a CTC isolation platform that simultaneously optimizes recovery rates, purity, and cell compatibility. Herein, a novel tracheal carina-inspired bifurcated (TRAB) microfilter system is reported, which uses an optimal filter gap size satisfying both 100% theoretical recovery rate and purity, as determined by biomechanical analysis and fluid–structure interaction (FSI) simulations. Biomechanical properties are also used to clearly discriminate between cancer cells and leukocytes, whereby cancer cells are selectively bound to melamine microbeads, which increase the size and stiffness of these cells. Nanoindentation experiments are conducted to measure the stiffness of leukocytes as compared to the microbead-conjugated cancer cells, with these parameters then being used in FSI analyses to optimize the filter gap size. The simulation results show that given a flow rate of 100 μL min−1, an 8 μm filter gap optimizes the recovery rate and purity. MCF-7 breast cancer cells with solid microbeads are spiked into 3 mL of whole blood and, by using this flow rate along with the optimized microfilter dimensions, the cell mixture passes through the TRAB filter, which achieves a recovery rate of 93% and purity of 59%. Regarding cell compatibility, it is verified that the isolation procedure does not adversely affect cell viability, thus also confirming that the re-collected cancer cells can be cultured for up to 8 days. This work demonstrates a CTC isolation technology platform that optimizes high recovery rates and cell purity while also providing a framework for functional cell studies, potentially enabling even more sensitive and specific cancer diagnostics.
Full Text
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Seung Il(김승일)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.