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Association between VEGF and eNOS gene polymorphisms and lumbar disc degeneration in a young Korean population

Authors
 I.B. Han  ;  A.E. Ropper  ;  Y.D. Teng  ;  D.A. Shin  ;  Y.J. Jeon  ;  H.M. Park  ;  D.E. Shin  ;  Y.S. Park  ;  K.N. Kim  ;  N.-K. Kim 
Citation
 GENETICS AND MOLECULAR RESEARCH , Vol.12(3) : 2294-2305, 2013 
Journal Title
GENETICS AND MOLECULAR RESEARCH
Issue Date
2013
MeSH
Adolescent ; Adult ; Back Pain/genetics ; Case-Control Studies ; Female ; Genetic Association Studies ; Haplotypes ; Humans ; Intervertebral Disc Degeneration/genetics* ; Intervertebral Disc Displacement/genetics* ; Male ; Nitric Oxide Synthase Type III/genetics* ; Polymorphism, Restriction Fragment Length ; Polymorphism, Single Nucleotide* ; Population/genetics* ; Republic of Korea ; Vascular Endothelial Growth Factor A/genetics*
Keywords
Endothelial nitric oxide synthase ; Polymorphism ; Intervertebral disc degeneration ; Vascular endothelial growth factor
Abstract
Disturbances in blood flow to intervertebral discs (IVD) play an important role in IVD degeneration. Vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) are extremely important angiogenic factors for vasodilation and neovascularization. We investigated the relationship between single nucleotide polymorphisms (SNPs) of the VEGF and eNOS genes and genetic susceptibility to lumbar IVD degeneration in a young adult Korean population. Two hundred and forty-one participants (aged 18 to 30 years), with or without low back pain, were selected for the study. Magnetic resonance imaging was made of the lumbar spine in all participants. The patient group (N = 102) had low back pain clinically and lumbar IVD degeneration radiographically. The control group (N = 139) included subjects with and without low back pain; all were negative radiographically for lumbar IVD degeneration. Using PCR-RFLP analysis, we analyzed VEGF (-2578C>A, -1154G>A, -634G>C, and 936C>T) and eNOS (-786T>C, 4a4b and 894G>T) SNPs. We made combined analyses of the genes and performed haplotype analyses. There were no significant differences in the genotype distribution of polymorphisms of VEGF and eNOS genes among patients and controls. However, the frequency of VEGF -2578CA +AA/-634CC combined genotypes was significantly higher in patients when compared with controls [odds ratio (OR) = 21.00; 95% confidence interval (CI) = 2.590- 170.240]. The frequencies of the -2578A/-1154A/-634C/936C (OR = 3.831; 95%CI = 1.068-13.742), -2578A/-1154A/-634C (OR = 3.356; 95%CI = 1.198-9.400), and -2578A/-634C/936C (OR = 10.820; 95%CI = 2.811-41.656) haplotypes were also significantly higher in patients than in controls. We conclude that the combined genotype VEGF -2578CA+AA/-634CC is a possible risk factor for IVD degeneration and the VEGF -2578A/-1154A/-634C/936C haplotype may increase the risk for development of IVD degeneration. Furthermore, the VEGF -634C allele appears to be associated with susceptibility to IVD degeneration.
Files in This Item:
T201305409.pdf Download
DOI
10.4238/2013.July.8.10
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Shin, Dong Ah(신동아) ORCID logo https://orcid.org/0000-0002-5225-4083
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/88981
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