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Expression of the chemokine decoy receptor D6 mediates dendritic cell function and promotes corneal allograft rejection
DC Field | Value | Language |
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dc.contributor.author | 이형근 | - |
dc.date.accessioned | 2014-12-18T09:47:57Z | - |
dc.date.available | 2014-12-18T09:47:57Z | - |
dc.date.issued | 2013 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/88851 | - |
dc.description.abstract | PURPOSE: To identify the role of chemokine receptor 6 (D6) expression by dendritic cells (DCs) and its role in corneal transplant immunity. METHODS: Flow cytometry analysis was used to assess the expression level of the D6 chemokine receptor in different leukocyte populations and DC maturation following lipopolysaccharides (LPS) stimulation of bone marrow-derived DCs isolated from wild-type (WT) or D6(-/-) mice (C57BL/6 background). Mixed-lymphocyte reactions and delayed-type hypersensitivity assays were performed with bone marrow-derived DCs from WT or D6(-/-) mice to evaluate T-cell alloreactivity. Adoptive transfer experiments with T cells from WT or D6(-/-) hosts with BALB/c corneal allografts were performed. Graft opacity was assessed over an 8-week period, and graft survival was plotted using Kaplan-Meier survival curves. RESULTS: Expression of the D6 chemokine receptor was significantly higher in DCs compared to other leukocyte subpopulations, including neutrophils, lymphocytes, and monocytes/macrophages. LPS challenge of D6(-/-) bone marrow-derived DCs elicited significantly lower levels of major histocompatibility complex II and costimulatory molecules (CD40, CD80, and CD86) compared to WT bone marrow-derived DCs, indicating the role of the D6 chemokine receptor in DC maturation. Further, DCs isolated from D6(-/-) mice induced less T-cell proliferation (p≤0.001) and interferon-gamma production in T cells of draining lymph nodes compared to WT mice following corneal transplantation (p≤0.001). Moreover, adoptively transferred T cells from D6(-/-) corneal transplanted mice to WT mice led to impaired graft rejection, compared to the hosts that received T cells from the WT transplanted mice. CONCLUSIONS: We demonstrated D6 chemokine receptor expression by DCs and identified its critical function in multiple aspects of DC biology, including maturation and consequent elicitation of alloreactive T-cell responses that are responsible for corneal allograft rejection. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | MOLECULAR VISION | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adoptive Transfer | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antigens, CD/genetics | - |
dc.subject.MESH | Antigens, CD/immunology | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Cornea/immunology | - |
dc.subject.MESH | Cornea/pathology | - |
dc.subject.MESH | Cornea/surgery | - |
dc.subject.MESH | Corneal Transplantation* | - |
dc.subject.MESH | Dendritic Cells/drug effects | - |
dc.subject.MESH | Dendritic Cells/immunology* | - |
dc.subject.MESH | Dendritic Cells/pathology | - |
dc.subject.MESH | Gene Expression/immunology* | - |
dc.subject.MESH | Graft Rejection/genetics* | - |
dc.subject.MESH | Graft Rejection/immunology | - |
dc.subject.MESH | Graft Rejection/pathology | - |
dc.subject.MESH | Graft Survival/genetics* | - |
dc.subject.MESH | Graft Survival/immunology | - |
dc.subject.MESH | Hypersensitivity, Delayed/immunology | - |
dc.subject.MESH | Lipopolysaccharides/pharmacology | - |
dc.subject.MESH | Lymphocyte Culture Test, Mixed | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred BALB C | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Mice, Knockout | - |
dc.subject.MESH | Receptors, Chemokine/genetics* | - |
dc.subject.MESH | Receptors, Chemokine/immunology | - |
dc.subject.MESH | T-Lymphocytes/immunology | - |
dc.subject.MESH | T-Lymphocytes/transplantation | - |
dc.subject.MESH | Transplantation, Homologous | - |
dc.title | Expression of the chemokine decoy receptor D6 mediates dendritic cell function and promotes corneal allograft rejection | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학) | - |
dc.contributor.googleauthor | Amir R. Hajrasouliha | - |
dc.contributor.googleauthor | Zahra Sadrai | - |
dc.contributor.googleauthor | Hyung K. Lee | - |
dc.contributor.googleauthor | Sunil K. Chauhan | - |
dc.contributor.googleauthor | Reza Dana | - |
dc.identifier.doi | 24357920 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03303 | - |
dc.relation.journalcode | J02272 | - |
dc.identifier.eissn | 1090-0535 | - |
dc.identifier.pmid | 24357920 | - |
dc.subject.keyword | Adoptive Transfer | - |
dc.subject.keyword | Animals | - |
dc.subject.keyword | Antigens, CD/genetics | - |
dc.subject.keyword | Antigens, CD/immunology | - |
dc.subject.keyword | Cell Proliferation | - |
dc.subject.keyword | Cells, Cultured | - |
dc.subject.keyword | Cornea/immunology | - |
dc.subject.keyword | Cornea/pathology | - |
dc.subject.keyword | Cornea/surgery | - |
dc.subject.keyword | Corneal Transplantation* | - |
dc.subject.keyword | Dendritic Cells/drug effects | - |
dc.subject.keyword | Dendritic Cells/immunology* | - |
dc.subject.keyword | Dendritic Cells/pathology | - |
dc.subject.keyword | Gene Expression/immunology* | - |
dc.subject.keyword | Graft Rejection/genetics* | - |
dc.subject.keyword | Graft Rejection/immunology | - |
dc.subject.keyword | Graft Rejection/pathology | - |
dc.subject.keyword | Graft Survival/genetics* | - |
dc.subject.keyword | Graft Survival/immunology | - |
dc.subject.keyword | Hypersensitivity, Delayed/immunology | - |
dc.subject.keyword | Lipopolysaccharides/pharmacology | - |
dc.subject.keyword | Lymphocyte Culture Test, Mixed | - |
dc.subject.keyword | Male | - |
dc.subject.keyword | Mice | - |
dc.subject.keyword | Mice, Inbred BALB C | - |
dc.subject.keyword | Mice, Inbred C57BL | - |
dc.subject.keyword | Mice, Knockout | - |
dc.subject.keyword | Receptors, Chemokine/genetics* | - |
dc.subject.keyword | Receptors, Chemokine/immunology | - |
dc.subject.keyword | T-Lymphocytes/immunology | - |
dc.subject.keyword | T-Lymphocytes/transplantation | - |
dc.subject.keyword | Transplantation, Homologous | - |
dc.contributor.alternativeName | Lee, Hyung Keun | - |
dc.contributor.affiliatedAuthor | Lee, Hyung Keun | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 19 | - |
dc.citation.startPage | 2517 | - |
dc.citation.endPage | 2525 | - |
dc.identifier.bibliographicCitation | MOLECULAR VISION, Vol.19 : 2517-2525, 2013 | - |
dc.identifier.rimsid | 33665 | - |
dc.type.rims | ART | - |
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