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Loss of blaVIM-2 and blaIMP-1 during the Storage of Gram-Negative Bacilli, Antimicrobial Susceptibility of the Gene-Lost Strain, and Location of the Gene in the Cell.

DC Field Value Language
dc.contributor.author서영희-
dc.contributor.author용동은-
dc.contributor.author이경원-
dc.contributor.author이양순-
dc.contributor.author정윤섭-
dc.date.accessioned2014-12-18T09:47:07Z-
dc.date.available2014-12-18T09:47:07Z-
dc.date.issued2013-
dc.identifier.issn2288-0585-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/88824-
dc.description.abstractBackground Gram-negative bacilli can be stored in cystine tryptic agar (CTA) at room temperature for over 1 year, but we experienced a loss of imipenem resistance among VIM-2-producing isolates. The aims of this study were to determine the frequency of loss of IMP-1 and VIM-2 genes during storage in CTA at room temperature and to document any change in the MIC of antimicrobial agents and the location of the gene. Methods Bacteria were isolated from clinical specimens at Severance Hospital collected from 1995-2000. Modified Hodge and double disk synergy tests were performed for screening of MBL-production isolates, and blaIMP-1 and blaVIM-2 were detected by PCR. Loss of resistance was tested in CTA at room temperature. PFGE and hybridization using a blaVIM-2 probe were carried out to determine the location of the VIM-2 gene. Results When VIM-2- and IMP-1-producing strains of eight P. aeruginosa and two Acinetobacter spp. were stored in CTA at room temperature, some isolates lost imipenem resistance after 3 days and 90% lost resistance after 15 weeks. Loss of resistance genes resulted in a decrease of the MIC of imipenem from 32-128 μg/mL to 0.5-8 μg/mL for P. aeruginosa, and from 32 μg/mL to 0.25-4 μg/mL for Acinetobacter spp. Hybridization of I-CeuI and S1-digested and PFGE suggested that VIM-2 genes are located on approximately 50-100 kb or 400 kb plasmids. Conclusion Isolates may lose resistance genes when stored in CTA at room temperature. Therefore, it is necessary for MBL-production tests including the Modified Hodge test and double disk synergy test and detection of MBL genes.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfAnnals of Clinical Microbiology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleLoss of blaVIM-2 and blaIMP-1 during the Storage of Gram-Negative Bacilli, Antimicrobial Susceptibility of the Gene-Lost Strain, and Location of the Gene in the Cell.-
dc.title.alternative그람음성 막대균 보존 중 blaVIM-2 및 blaIMP-1 유전자의 소실, 소실 균주의 항균제 감수성 및 유전자의 위치-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthorYoungsik Lim-
dc.contributor.googleauthorYangsoon Lee-
dc.contributor.googleauthorYounghee Seo-
dc.contributor.googleauthorJong Hwa Yum-
dc.contributor.googleauthorDongeun Yong-
dc.contributor.googleauthorKyungwon Lee-
dc.contributor.googleauthorYunsop Chong-
dc.identifier.doi10.5145/ACM.2013.16.3.120-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01895-
dc.contributor.localIdA02423-
dc.contributor.localIdA02649-
dc.contributor.localIdA02947-
dc.contributor.localIdA03679-
dc.relation.journalcodeJ00156-
dc.identifier.pmidPrecocious puberty ; Diagnosis ; Gonadotropin-releasing hormone ; Forecasting ; Female-
dc.subject.keywordPrecocious puberty-
dc.subject.keywordDiagnosis-
dc.subject.keywordGonadotropin-releasing hormone-
dc.subject.keywordForecasting-
dc.subject.keywordFemale-
dc.contributor.alternativeNameSeo, Young Hee-
dc.contributor.alternativeNameYong, Dong Eun-
dc.contributor.alternativeNameLee, Kyung Won-
dc.contributor.alternativeNameLee, Yang Soon-
dc.contributor.alternativeNameChong, Yun Sop-
dc.contributor.affiliatedAuthorSeo, Young Hee-
dc.contributor.affiliatedAuthorYong, Dong Eun-
dc.contributor.affiliatedAuthorLee, Kyung Won-
dc.contributor.affiliatedAuthorLee, Yang Soon-
dc.contributor.affiliatedAuthorChong, Yun Sop-
dc.rights.accessRightsfree-
dc.citation.volume16-
dc.citation.number3-
dc.citation.startPage120-
dc.citation.endPage125-
dc.identifier.bibliographicCitationAnnals of Clinical Microbiology, Vol.16(3) : 120-125, 2013-
dc.identifier.rimsid33644-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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