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Role of Endogenous ENaC and TRP Channels in the Myogenic Response of Rat Posterior Cerebral Arteries

Authors
 Eok-Cheon Kim  ;  Soo-Kyoung Choi  ;  Mihwa Lim  ;  Soo-In Yeon  ;  Young-Ho Lee 
Citation
 PLOS ONE, Vol.8(12) : e84194, 2013 
Journal Title
 PLOS ONE 
Issue Date
2013
MeSH
Amiloride ; Animals ; Blood Pressure/physiology* ; Epithelial Sodium Channels/metabolism* ; Hemodynamics/physiology* ; Imidazoles ; Phenanthrenes ; Posterior Cerebral Artery/physiology* ; RNA, Small Interfering ; Rats ; TRPM Cation Channels/metabolism*
Keywords
Amiloride ; Animals ; Blood Pressure/physiology* ; Epithelial Sodium Channels/metabolism* ; Hemodynamics/physiology* ; Imidazoles ; Phenanthrenes ; Posterior Cerebral Artery/physiology* ; RNA, Small Interfering ; Rats ; TRPM Cation Channels/metabolism*
Abstract
AIMS: Mechanogated ion channels are predicted to mediate pressure-induced myogenic vasoconstriction in small resistance arteries. Recent findings have indicated that transient receptor potential (TRP) channels and epithelial sodium channels (ENaC) are involved in mechanotransduction. The purpose of this study was to investigate the role of TRP channels and ENaC in the myogenic response. Our previous study suggested that ENaC could be a component of the mechanosensitive ion channels in rat posterior cerebral arteries (PCA). However, the specific ion channel proteins mediating myogenic constriction are unknown. Here we found, for the first time, that ENaC interacted with TRPM4 but not with TRPC6 using immunoprecipitation and confocal microscopy. METHODS AND RESULTS: Treatment with a specific βENaC inhibitor, amiloride, a specific TRPM4 inhibitor, 9-phenanthrol, and a TRPC6 inhibitor, SKF96365, resulted in inhibition of the pressure-induced myogenic response. Moreover, the myogenic response was inhibited in rat PCA transfected with small interfering RNA of βENaC, TRPM4, and TRPC6. Co-treatment with amiloride and 9-phenanthrol showed a similar inhibitory effect on myogenic contraction compared to single treatment with amiloride or 9-phenanthrol. The myogenic response was not affected by 9-phenanthrol or amiloride treatment in PCA transfected with βENaC or TRPM4 siRNA, respectively. However, pressure-induced myogenic response was fully inhibited by co-treatment with amiloride, 9-phenanthrol, and SKF96365, and by treatment with SKF96365 in PCA transfected with βENaC siRNA. CONCLUSION: Our results suggest that ENaC, TRPM4, and TRPC6 play important roles in the pressure-induced myogenic response, and that ENaC and TRPM4 interact in rat PCA.
Files in This Item:
T201304536.pdf Download
DOI
10.1371/journal.pone.0084194
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Yeon, Soo In(연수인)
Lee, Young Ho(이영호) ORCID logo https://orcid.org/0000-0002-5749-1045
Lim, Mi Hwa(임미화)
Choi, Soo Kyoung(최수경) ORCID logo https://orcid.org/0000-0002-7115-6358
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/88726
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