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A novel human anti-VCAM-1 monoclonal antibody ameliorates airway inflammation and remodelling

DC FieldValueLanguage
dc.contributor.author박중원-
dc.contributor.author손정호-
dc.contributor.author이재현-
dc.date.accessioned2014-12-18T09:41:51Z-
dc.date.available2014-12-18T09:41:51Z-
dc.date.issued2013-
dc.identifier.issn1582-1838-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/88656-
dc.description.abstractAsthma is a chronic inflammatory disease induced by Type 2 helper T cells and eosinophils. Vascular cell adhesion molecule-1 (VCAM-1) has been implicated in recruiting eosinophils and lymphocytes to pathological sites in asthma as a regulatory receptor. Accordingly, monoclonal antibody (mAb) against VCAM-1 may attenuate allergic inflammation and pathophysiological features of asthma. We attempted to evaluate whether a recently developed human anti-VCAM-1 mAb can inhibit the pathophysiological features of asthma in a murine asthma model induced by ovalbumin (OVA). Leucocyte adhesion inhibition assay was performed to evaluate the in vitro blocking activity of human anti-VCAM-1 mAb. OVA-sensitized BALB/c mice were treated with human anti-VCAM-1 mAb or isotype control Ab before intranasal OVA challenge. We evaluated airway hyperresponsiveness (AHR) and bronchoalveolar lavage fluid analysis, measured inflammatory cytokines and examined histopathological features. The human anti-VCAM-1 mAb bound to human and mouse VCAM-1 molecules and inhibited adhesion of human leucocytes in vitro. AHR and inflammatory cell counts in bronchoalveolar lavage fluid were reduced in mice treated with human anti-VCAM-1 mAb as compared with a control Ab. The levels of interleukin (IL)-5 and IL-13, as well as transforming growth factor-β, in lung tissue were decreased in treated mice. Human anti-VCAM-1 mAb reduced goblet cell hyperplasia and peribronchial fibrosis. In vivo VCAM-1 expression decreased in the treated group. In conclusion, human anti-VCAM-1 mAb attenuated allergic inflammation and the pathophysiological features of asthma in OVA-induced murine asthma model. The results suggested that human anti-VCAM-1 mAb could potentially be used as an additional anti-asthma therapeutic medicine.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJournal of Cellular and Molecular Medicine-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAntibodies, Monoclonal/immunology*-
dc.subject.MESHAsthma/immunology*-
dc.subject.MESHAsthma/therapy-
dc.subject.MESHHumans-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred BALB C-
dc.subject.MESHVascular Cell Adhesion Molecule-1/immunology*-
dc.titleA novel human anti-VCAM-1 monoclonal antibody ameliorates airway inflammation and remodelling-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorJae-Hyun Lee-
dc.contributor.googleauthorJung-Ho Sohn-
dc.contributor.googleauthorSu Yeon Ryu-
dc.contributor.googleauthorChein-Soo Hong-
dc.contributor.googleauthorKyung D. Moon-
dc.contributor.googleauthorJung-Won Park-
dc.identifier.doi10.1111/jcmm.12102-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01681-
dc.contributor.localIdA01993-
dc.contributor.localIdA03086-
dc.relation.journalcodeJ01302-
dc.identifier.pmid23855490-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/jcmm.12102/abstract-
dc.subject.keywordVCAM-1-
dc.subject.keywordallergic inflammation-
dc.subject.keywordanti-inflammatory effect-
dc.subject.keywordasthma-
dc.subject.keywordcell adhesion molecule-
dc.subject.keywordmonoclonal antibody-
dc.contributor.alternativeNamePark, Jung Won-
dc.contributor.alternativeNameSohn, Jung Ho-
dc.contributor.alternativeNameLee, Jae Hyun-
dc.contributor.affiliatedAuthorPark, Jung Won-
dc.contributor.affiliatedAuthorSohn, Jung Ho-
dc.contributor.affiliatedAuthorLee, Jae Hyun-
dc.rights.accessRightsnot free-
dc.citation.volume17-
dc.citation.number10-
dc.citation.startPage1271-
dc.citation.endPage1281-
dc.identifier.bibliographicCitationJournal of Cellular and Molecular Medicine, Vol.17(10) : 1271-1281, 2013-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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