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The Inhibitory Effect of Buddlejasaponin IV on the Growth of YD-10B Human Oral Squamous Cell Carcinoma Cells
DC Field | Value | Language |
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dc.contributor.author | 김기림 | - |
dc.contributor.author | 박광균 | - |
dc.contributor.author | 정원윤 | - |
dc.contributor.author | 황영선 | - |
dc.date.accessioned | 2014-12-18T09:41:00Z | - |
dc.date.available | 2014-12-18T09:41:00Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 2288-3649 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/88630 | - |
dc.description.abstract | Background: Buddlejasaponin IV (BS-IV), a triterpene saponin isolated from Pleurospermum kamtschaticum HOFFMANN (Umbelliferae), is known to have potent anti-inflammatory activity and cytotoxicity against diverse cancer cell lines. In the present study, we attempted to verify whether BS-IV could inhibit cell growth, and induce cell cycle arrest and apoptosis in highly invasive YD-10B human oral squamous cell carcinoma (OSCC) cells. Methods: YD-10B cells were treated with various concentrations of BS-IV, and the cell viability was evaluated by MTT assay. Flow cytometry was conducted to examine cell phase distribution and DAPI staining was performed to observe apoptotic morphological changes in BS-IV-treated YD-10B cells. Western blot analysis was used to investigate the expression of proteins associated with cell cycle arrest and apoptosis.Results: BS-IV treatment significantly reduced the viability of YD-10B cells and partially arrested cell cycle progression at the G2/M phase. Treatment with BS-IV substantially decreased the levels of cyclin B1 and stimulated the phosphorylation of checkpoint kinase 2 (Chk2). The expression of p21 was increased but the phosphorylation of Akt was inhibited in BS-IV-treated YD-10B cells. Furthermore, BS-IV induced release of cytochrome c from mitochondria by reducing anti-apoptotic Bcl-2 level and increasing pro-apoptotic Bax level. Active caspase-3 level and the cleavage of poly (ADP-ribose) polymerase (PARP) were enhanced by BS-IV treatment. In addition, BS-IV increased the expression of Fas death receptor and its ligand (FasL) in YD-10B cells. Conclusions: The treatment with BS-IV inhibits the growth of YD-10B cells by inducing p21-dependent cell cycle arrest at G2/M phase and apoptosis through both mitochondrial-dependent and death receptor-mediated pathways. Thus, BS-IV is an excellent candidate for a chemopreventive agent to block the progression of human OSCC. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | JOURNAL OF CANCER PREVENTION | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | The Inhibitory Effect of Buddlejasaponin IV on the Growth of YD-10B Human Oral Squamous Cell Carcinoma Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Ki-Rim Kim | - |
dc.contributor.googleauthor | Kwang-Kyun Park | - |
dc.contributor.googleauthor | Won-Yoon Chung | - |
dc.contributor.googleauthor | Young Sun Hwang | - |
dc.identifier.doi | 25337562 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00333 | - |
dc.contributor.localId | A01429 | - |
dc.contributor.localId | A03676 | - |
dc.contributor.localId | A04472 | - |
dc.relation.journalcode | J01282 | - |
dc.identifier.eissn | 2288-3657 | - |
dc.identifier.pmid | 25337562 | - |
dc.subject.keyword | Apoptosis | - |
dc.subject.keyword | Buddlejasaponin IV | - |
dc.subject.keyword | Cell cycle arrest | - |
dc.subject.keyword | Oral squamous cell carcinoma | - |
dc.contributor.alternativeName | Kim, Ki Rim | - |
dc.contributor.alternativeName | Park, Kwang Kyun | - |
dc.contributor.alternativeName | Chung, Won Yoon | - |
dc.contributor.alternativeName | Hwang, Young Sun | - |
dc.contributor.affiliatedAuthor | Kim, Ki Rim | - |
dc.contributor.affiliatedAuthor | Park, Kwang Kyun | - |
dc.contributor.affiliatedAuthor | Chung, Won Yoon | - |
dc.contributor.affiliatedAuthor | Hwang, Young Sun | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 18 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 330 | - |
dc.citation.endPage | 336 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CANCER PREVENTION, Vol.18(4) : 330-336, 2013 | - |
dc.identifier.rimsid | 33368 | - |
dc.type.rims | ART | - |
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