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Comparison of matrix-assisted laser desorption ionization–time-of-flight mass spectrometry assay with conventional methods for detection of IMP-6, VIM-2, NDM-1, SIM-1, KPC-1, OXA-23, and OXA-51 carbapenemase-producing Acinetobacter spp., Pseudomonas aeruginosa, and Klebsiella pneumoniae

Authors
 Wonmok Lee ; Hae-Sun Chung ; Yunsop Chong ; Kyungwon Lee ; Seok Hoon Jeong ; Dongeun Yong ; Yangsoon Lee 
Citation
 Diagnostic Microbiology and Infectious Disease, Vol.77(3) : 227~230, 2013 
Journal Title
 Diagnostic Microbiology and Infectious Disease 
ISSN
 0732-8893 
Issue Date
2013
Abstract
Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay was able to detect carbapenemase producers, including SIM-1 or OXA-51, within 4 hours using 20 μL of 0.5 g/L ertapenem solution as a substrate. This assay is more rapid and accurate than the modified Hodge test and 3-dimensional extract bioassay. Hence, it can be used an alternative test to identify carbapenemase-mediated carbapenem resistance in Gram-negative bacteria.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/88292
DOI
10.1016/j.diagmicrobio.2013.07.005
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Laboratory Medicine
Yonsei Authors
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Link
 http://www.sciencedirect.com/science/article/pii/S0732889313003982
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