Cited 4 times in
Quantitation and pharmacokinetics of 1,4-diamino-2,3-dicyano-1,4-bis (2-aminophenylthio) butadiene (U0126) in rat plasma by liquid chromatography-tandem mass spectrometry
DC Field | Value | Language |
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dc.contributor.author | 신상준 | - |
dc.contributor.author | 황지원 | - |
dc.date.accessioned | 2014-12-18T09:21:42Z | - |
dc.date.available | 2014-12-18T09:21:42Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1615-9306 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/88028 | - |
dc.description.abstract | A simple, robust, and rapid LC-MS/MS method was developed for the quantitation of U0126 and validated in rat plasma. Plasma samples (20 μL) were deproteinized using 200 μL ACN containing 30 ng/mL of chlorpropamide, internal standard. Chromatographic separation performed on an Agilent Poroshell 120 EC-C(18) column (4.6 × 50 mm, 2.7 μm particle size) with an isocratic mobile phase consisting of a 70:30 v/v mixture of ACN and 0.1% aqueous formic acid. Each sample was run at 0.6 mL/min for a total run time of 2 min per sample. Detection and quantification were performed using a mass spectrometer in selected reaction-monitoring mode with positive ESI at m/z 381 → 123.9 for U0126 and m/z 277 → 175 for the internal standard. The standard curve was linear over a concentration range of 20-5000 ng/mL with correlation coefficients greater than 0.9965. Precision, both intra- and interday, was less than 10.1% with an accuracy of 90.7-99.4%. No matrix effects were observed. U0126 in rat plasma degraded approximately 41.3% after 3-h storage at room temperature. To prevent degradation, sample handling should be on an ice bath and all solutions kept at 4°C. This method was successfully applied to a pharmacokinetic study of U0126 at various doses in rats | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | JOURNAL OF SEPARATION SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Butadienes/blood | - |
dc.subject.MESH | Butadienes/pharmacokinetics* | - |
dc.subject.MESH | Chromatography, Liquid/methods* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Nitriles/blood | - |
dc.subject.MESH | Nitriles/pharmacokinetics* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Tandem Mass Spectrometry/methods* | - |
dc.title | Quantitation and pharmacokinetics of 1,4-diamino-2,3-dicyano-1,4-bis (2-aminophenylthio) butadiene (U0126) in rat plasma by liquid chromatography-tandem mass spectrometry | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | Soo Hyeon Bae | - |
dc.contributor.googleauthor | Jee Won Hwang | - |
dc.contributor.googleauthor | Sang Joon Shin | - |
dc.contributor.googleauthor | Gyu Hwan Park | - |
dc.contributor.googleauthor | Kee Dong Yoon | - |
dc.contributor.googleauthor | Soo Kyung Bae | - |
dc.identifier.doi | 10.1002/jssc.201200779 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02105 | - |
dc.contributor.localId | A04490 | - |
dc.relation.journalcode | J01746 | - |
dc.identifier.eissn | 1615-9314 | - |
dc.identifier.pmid | 23225735 | - |
dc.identifier.url | http://dx.doi.org/10.1002/jssc.201200779 | - |
dc.subject.keyword | 1,4-Diamino-2,3-dicyano-1,4-bis (2-aminophenylthio) butadiene(U0126) | - |
dc.subject.keyword | Liquid chromatography-tandem mass spectrometry | - |
dc.subject.keyword | Rat plasma | - |
dc.subject.keyword | Validation | - |
dc.contributor.alternativeName | Shin, Sang Joon | - |
dc.contributor.alternativeName | Hwang, Jee Won | - |
dc.contributor.affiliatedAuthor | Shin, Sang Joon | - |
dc.contributor.affiliatedAuthor | Hwang, Jee Won | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 36 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 239 | - |
dc.citation.endPage | 245 | - |
dc.identifier.bibliographicCitation | JOURNAL OF SEPARATION SCIENCE, Vol.36(2) : 239-245, 2013 | - |
dc.identifier.rimsid | 32711 | - |
dc.type.rims | ART | - |
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