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Antibody-Secreting Cells with a Phenotype of Ki-67(low), CD138(high), CD31(high), and CD38(high) Secrete Nonspecific IgM during Primary Hepatitis A Virus Infection

Authors
 Seokchan Hong  ;  Hyun Woong Lee  ;  Dong-Yeop Chang  ;  Sooseong You  ;  Jihye Kim  ;  Jun Yong Park  ;  Sang Hoon Ahn  ;  Dongeun Yong  ;  Kwang-Hyub Han  ;  Ook Joon Yoo  ;  Eui-Cheol Shin 
Citation
 JOURNAL OF IMMUNOLOGY, Vol.191(1) : 127-134, 2013 
Journal Title
 JOURNAL OF IMMUNOLOGY 
ISSN
 0022-1767 
Issue Date
2013
MeSH
ADP-ribosyl Cyclase 1/biosynthesis* ; Acute Disease ; Adult ; Antibody Specificity ; Antibody-Producing Cells/immunology* ; Antibody-Producing Cells/secretion ; Antibody-Producing Cells/virology ; Hepatitis A/immunology* ; Hepatitis A/metabolism ; Hepatitis A/pathology* ; Humans ; Immunoglobulin M/biosynthesis* ; Immunophenotyping ; Ki-67 Antigen/biosynthesis ; Ki-67 Antigen/metabolism* ; Leukocyte Count ; Leukocytes, Mononuclear/immunology ; Leukocytes, Mononuclear/metabolism ; Leukocytes, Mononuclear/pathology ; Membrane Glycoproteins/biosynthesis* ; Platelet Endothelial Cell Adhesion Molecule-1/biosynthesis* ; Syndecan-1/biosynthesis* ; Young Adult
Keywords
ADP-ribosyl Cyclase 1/biosynthesis* ; Acute Disease ; Adult ; Antibody Specificity ; Antibody-Producing Cells/immunology* ; Antibody-Producing Cells/secretion ; Antibody-Producing Cells/virology ; Hepatitis A/immunology* ; Hepatitis A/metabolism ; Hepatitis A/pathology* ; Humans ; Immunoglobulin M/biosynthesis* ; Immunophenotyping ; Ki-67 Antigen/biosynthesis ; Ki-67 Antigen/metabolism* ; Leukocyte Count ; Leukocytes, Mononuclear/immunology ; Leukocytes, Mononuclear/metabolism ; Leukocytes, Mononuclear/pathology ; Membrane Glycoproteins/biosynthesis* ; Platelet Endothelial Cell Adhesion Molecule-1/biosynthesis* ; Syndecan-1/biosynthesis* ; Young Adult
Abstract
Although studies investigating the nature of Ab-secreting cells (ASCs) during acute infection with influenza or dengue virus found that the ASC response was dominated by virus-specific IgG secretion, the Ag specificity and phenotype of ASCs during primary acute viral infection were not identified. To this end, we investigated the nature of ASCs in direct ex vivo assays from patients with acute hepatitis A caused by primary infection with hepatitis A virus (HAV). We found that the frequency of CD27(high)CD38(high) ASCs was markedly increased in the peripheral blood during the acute phase of HAV infection. Moreover, substantial numbers of ASCs were non-HAV-specific and dominantly secreted IgM. We detected HAV-specific ASCs by staining with fluorochrome-tagged HAV-VP1 protein. As compared with HAV-specific ASCs, non-HAV-specific ASCs were Ki-67(low)CD138(high)CD31(high)CD38(high), demonstrating that non-HAV-specific ASCs had a bone marrow plasma cell-like phenotype whereas HAV-specific ASCs had a phenotype typical of circulating plasmablasts. These data suggest that non-HAV-specific ASCs might be mobilized plasma cells from the bone marrow or the spleen, whereas HAV-specific ASCs were newly generated plasmablasts. In this study, we provide evidence that pre-existing plasma cells are released into the circulation and contribute to Ag-nonspecific secretion of IgM during primary HAV infection.
Full Text
http://www.jimmunol.org/content/191/1/127.long
DOI
10.4049/jimmunol.1203540
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Park, Jun Yong(박준용) ORCID logo https://orcid.org/0000-0001-6324-2224
Ahn, Sang Hoon(안상훈) ORCID logo https://orcid.org/0000-0002-3629-4624
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Han, Kwang-Hyub(한광협) ORCID logo https://orcid.org/0000-0003-3960-6539
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87973
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