0 142

Cited 9 times in

The expression of glutamine-metabolism-related proteins in breast phyllodes tumors

Authors
 Sewha Kim  ;  Woo Hee Jung  ;  Ja Seung Koo 
Citation
 TUMOR BIOLOGY, Vol.34(5) : 2683-2689, 2013 
Journal Title
 TUMOR BIOLOGY 
ISSN
 1010-4283 
Issue Date
2013
MeSH
Adult ; Amino Acid Transport System ASC/metabolism* ; Breast Neoplasms/metabolism* ; Breast Neoplasms/mortality ; Breast Neoplasms/pathology ; Disease-Free Survival ; Female ; Glutaminase/metabolism* ; Glutamine/metabolism ; Humans ; Kaplan-Meier Estimate ; Laser Capture Microdissection ; Middle Aged ; Minor Histocompatibility Antigens ; Neoplasm Grading ; Phyllodes Tumor/metabolism* ; Phyllodes Tumor/mortality ; Phyllodes Tumor/pathology ; Prognosis ; Retrospective Studies ; Sugar Alcohol Dehydrogenases/metabolism* ; Tissue Array Analysis
Keywords
Breast ; Phyllodes tumor ; Glutamine ; Metabolism
Abstract
The aim of this study was to investigate the expression of glutamine-metabolism-related proteins according to the histologic grade of phyllodes tumors (PTs) and to assess its clinical implication. We generated tissue microarrays of 224 PTs and performed immunohistochemical staining and western blot analysis of glutamine-metabolism-related molecules, including GLS1, GDH, and ASCT2. The associations between immunohistochemical results and clinicopathologic parameters were evaluated. The expression of GLS1 (p < 0.001), GDH (p < 0.001), and ASCT2 (p = 0.005) in stromal components significantly increased with worsening PT histological grade. GDH expression in epithelial components significantly increased in high-grade PT (p = 0.026). In western blot, stromal expression of GLS1, GDH, and ASCT2 increased as histologic grade increased. By univariate analysis, stromal GLS1 expression (p = 0.022) and stromal GDH expression (p = 0.009) were independent predictors of shorter DFS. Stromal GLS1 expression (p < 0.001) and stromal GDH expression (p < 0.001) were independent predictors of shorter OS. This study demonstrated that the stromal expression of the glutamine-metabolism-related proteins GLS1, GDH, ASCT2 increases with worsening histological PT grade.
Full Text
http://link.springer.com/article/10.1007%2Fs13277-013-0819-7
DOI
10.1007/s13277-013-0819-7
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Koo, Ja Seung(구자승) ORCID logo https://orcid.org/0000-0003-4546-4709
Jung, Woo Hee(정우희)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87789
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse