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Standardization of multilocus sequence typing scheme for Mycobacterium abscessus and Mycobacterium massiliense

Authors
 Song Yee Kim  ;  Young Ae Kang  ;  Il Kwon Bae  ;  Jae-Joon Yim  ;  Moo Suk Park  ;  Young Sam Kim  ;  Se Kyu Kim  ;  Joon Chang  ;  Seok Hoon Jeong 
Citation
 DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, Vol.77(2) : 143-149, 2013 
Journal Title
 DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE 
ISSN
 0732-8893 
Issue Date
2013
MeSH
Bacterial Proteins/genetics ; DNA, Bacterial/analysis ; DNA, Bacterial/genetics ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Multilocus Sequence Typing/methods* ; Multilocus Sequence Typing/standards* ; Mycobacterium/classification* ; Mycobacterium/genetics ; Mycobacterium Infections/microbiology* ; Retrospective Studies
Keywords
Mycobacterium abscessus ; Mycobacterium massiliense ; Multilocus sequence analysis ; rpoB ; Multilocus sequence typing
Abstract
This study aims to develop a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus complex for the typing of stains within each species. A total of 89 clinical isolates of M. abscessus complex from 71 patients of 2 tertiary care hospitals in South Korea were included. Forty-two isolates were identified as M. abscessus, and 29, as Mycobacterium massiliense through sequencing of 8 housekeeping genes and rpoB. The MLST scheme identified 26 different sequence types(STs) and 13 different clonal complexes (CCs) in M. abscessus and 12 different STs and 6 different CCs in M. massiliense. The MLST data showed high concordance with the XbaI-macrorestriction patterns of pulsed-field gel electrophoresis in the duplicated isolates. Our MLST schemes could identify different strains of M. abscessus and M. massiliense, and the schemes also showed a reliable reproducibility. Therefore, our MLST schemes may be useful in studying the epidemiology of M. abscessus and M. massiliense infections.
Full Text
http://www.sciencedirect.com/science/article/pii/S0732889313003787
DOI
10.1016/j.diagmicrobio.2013.06.023
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Young Ae(강영애) ORCID logo https://orcid.org/0000-0002-7783-5271
Kim, Se Kyu(김세규)
Kim, Song Yee(김송이) ORCID logo https://orcid.org/0000-0001-8627-486X
Kim, Young Sam(김영삼) ORCID logo https://orcid.org/0000-0001-9656-8482
Park, Moo Suk(박무석) ORCID logo https://orcid.org/0000-0003-0820-7615
Bae, Il Kwon(배일권)
Chang, Joon(장준) ORCID logo https://orcid.org/0000-0003-4542-6841
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87654
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