4 204

Cited 18 times in

Silencing of Atp2b1 increases blood pressure through vasoconstriction.

Authors
 Young-Bin Shin  ;  Ji Eun Lim  ;  Su-Min Ji  ;  Hyeon-Ju Lee  ;  So-Yon Park  ;  Kyung-Won Hong  ;  Mihwa Lim  ;  Mark I. McCarthy  ;  Young-Ho Lee  ;  Bermseok Oh 
Citation
 JOURNAL OF HYPERTENSION, Vol.31(8) : 1575-1583, 2013 
Journal Title
 JOURNAL OF HYPERTENSION 
ISSN
 0263-6352 
Issue Date
2013
MeSH
Animals ; Blood Pressure ; Calcium/chemistry ; Gene Silencing* ; Genome-Wide Association Study ; Mice ; Mice, Inbred BALB C ; NIH 3T3 Cells ; Phenylephrine/chemistry ; Plasma Membrane Calcium-Transporting ATPases/genetics* ; RNA, Small Interfering/metabolism ; Real-Time Polymerase Chain Reaction ; Risk Factors ; Time Factors ; Vasoconstriction/genetics*
Keywords
ATP2B1 ; blood pressure ; siRNA ; vasoconstriction
Abstract
BACKGROUND: Recent genome-wide association studies (GWASs) have identified 30 genetic loci that regulate blood pressure, increasing our understanding of the cause of hypertension. However, it has been difficult to define the causative genes at these loci due to a lack of functional analyses. METHOD: In this study, we aimed to validate the candidate gene ATP2B1 in 12q21, variants near which have the strongest association with blood pressure in Asians and Europeans. ATP2B1 functions as a calcium pump to fine-tune calcium concentrations - necessary for repolarization following muscular contractions. We silenced Atp2b1 using an siRNA complex, injected into mouse tail veins. RESULTS: In treated mice, blood pressure rose and the mesenteric arteries increased in wall : lumen ratio. Moreover, the arteries showed enhanced myogenic responses to pressure, and contractile responses to phenylephrine increased compared with the control, suggesting that blood pressure is regulated by ATP2B1 through the contraction and dilation of the vessel, likely by controlling calcium concentrations in the resting state. CONCLUSION: These results support that ATP2B1 is the causative gene in the blood pressure-associated 12q21 locus and demonstrate that ATP2B1 expression in the vessel influences blood pressure.
Full Text
http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&AN=00004872-201308000-00014&LSLINK=80&D=ovft
DOI
10.1097/HJH.0b013e32836189e9
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Lee, Young Ho(이영호) ORCID logo https://orcid.org/0000-0002-5749-1045
Lim, Mi Hwa(임미화)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87621
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse