Cited 26 times in
Modulation of IL-8 Boosted by Mycoplasma pneumoniae lysate in Human Airway Epithelial Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김규언 | - |
dc.contributor.author | 손명현 | - |
dc.contributor.author | 이경은 | - |
dc.contributor.author | 홍정연 | - |
dc.contributor.author | 김경원 | - |
dc.date.accessioned | 2014-12-18T08:56:06Z | - |
dc.date.available | 2014-12-18T08:56:06Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0271-9142 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/87223 | - |
dc.description.abstract | Mycoplasma pneumoniae, a major cause of community-acquired pneumonia, has been recognized as a trigger for asthma inception and exacerbation. The epithelial cells on the respiratory tract parasitized by M. pneumoniae exhibit a number of cytopathic effects as a result of local inflammation and stimulated host immune response. We investigated the interactions of signaling molecules regulating the release of IL-8 by the direct stimulation of M. pneumoniae lysate (MPL) in human airway epithelial cells. In human airway epithelial cells, MPL-induced IL-8 proteins were decreased by monoclonal anti-TLR2 antibody in a dose-dependent fashion, and significantly blocked by siRNA TLR2. The pharmacologic inhibitors of ERK, U0126 and PD98059, effectively reduced IL-8 expression and the active forms of ERK signaling molecules, as detected by anti-phosphorylated p44/42 antibody. The region spanning from -132 to +41 in the IL-8 promoter demonstrated the highest luciferase activity against MPL and the mutations of NF-κB and NF-IL6 entirely diminished the activity. After investigating transfections of the NF-κB and NF-IL6 reporter vectors, NF-IL6 activation was significantly induced by MPL stimulation, which was considerably decreased by U0126 and monoclonal anti-TLR2 antibody. These results indicate that MPL-induced IL-8 increase is transcriptionally regulated by NF-IL6 more than by NF-κB. Additionally, the activation of NF-IL6 is influenced by TLR2 and ERK signaling pathways in airway epithelial cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | JOURNAL OF CLINICAL IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Antibodies, Blocking/pharmacology | - |
dc.subject.MESH | Antigens, Bacterial/immunology | - |
dc.subject.MESH | Butadienes/pharmacology | - |
dc.subject.MESH | CCAAT-Enhancer-Binding Protein-beta/genetics | - |
dc.subject.MESH | CCAAT-Enhancer-Binding Protein-beta/metabolism | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Flavonoids/pharmacology | - |
dc.subject.MESH | Gene Expression Regulation* | - |
dc.subject.MESH | Genetic Engineering | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interleukin-8/genetics* | - |
dc.subject.MESH | MAP Kinase Signaling System | - |
dc.subject.MESH | Mycoplasma pneumoniae/immunology* | - |
dc.subject.MESH | NF-kappa B/genetics | - |
dc.subject.MESH | NF-kappa B/metabolism | - |
dc.subject.MESH | Nitriles/pharmacology | - |
dc.subject.MESH | Promoter Regions, Genetic/genetics | - |
dc.subject.MESH | RNA, Small Interfering/genetics | - |
dc.subject.MESH | Respiratory Mucosa/drug effects | - |
dc.subject.MESH | Respiratory Mucosa/immunology* | - |
dc.subject.MESH | Toll-Like Receptor 2/genetics | - |
dc.subject.MESH | Toll-Like Receptor 2/immunology | - |
dc.subject.MESH | Toll-Like Receptor 2/metabolism* | - |
dc.subject.MESH | Transcriptional Activation/drug effects | - |
dc.subject.MESH | Transcriptional Activation/genetics | - |
dc.title | Modulation of IL-8 Boosted by Mycoplasma pneumoniae lysate in Human Airway Epithelial Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pediatrics (소아과학) | - |
dc.contributor.googleauthor | Kyung Eun Lee | - |
dc.contributor.googleauthor | Kyung Won Kim | - |
dc.contributor.googleauthor | Jung Yeon Hong | - |
dc.contributor.googleauthor | Kyu Earn Kim | - |
dc.contributor.googleauthor | Myung Hyun Sohn | - |
dc.identifier.doi | 10.1007/s10875-013-9909-y | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02651 | - |
dc.contributor.localId | A00327 | - |
dc.contributor.localId | A01967 | - |
dc.contributor.localId | A04431 | - |
dc.contributor.localId | A00303 | - |
dc.relation.journalcode | J01321 | - |
dc.identifier.eissn | 1573-2592 | - |
dc.identifier.pmid | 23779254 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs10875-013-9909-y | - |
dc.subject.keyword | Mycoplasma pneumoniae | - |
dc.subject.keyword | airway epithelial cell | - |
dc.subject.keyword | IL-8 | - |
dc.subject.keyword | TLR2 | - |
dc.subject.keyword | ERK | - |
dc.subject.keyword | NF-IL6 | - |
dc.contributor.alternativeName | Kim, Kyu Earn | - |
dc.contributor.alternativeName | Son, Myung Hyun | - |
dc.contributor.alternativeName | Lee, Kyung Eun | - |
dc.contributor.alternativeName | Hong, Jung Yeon | - |
dc.contributor.alternativeName | Kim, Kyung Won | - |
dc.contributor.affiliatedAuthor | Lee, Kyung Eun | - |
dc.contributor.affiliatedAuthor | Kim, Kyu Earn | - |
dc.contributor.affiliatedAuthor | Son, Myung Hyun | - |
dc.contributor.affiliatedAuthor | Hong, Jung Yeon | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Won | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 33 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1117 | - |
dc.citation.endPage | 1125 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CLINICAL IMMUNOLOGY, Vol.33(6) : 1117-1125, 2013 | - |
dc.identifier.rimsid | 32911 | - |
dc.type.rims | ART | - |
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