Cited 1 times in
A Method for Electroporation to Study Gene Function in Mammary Gland Development
DC Field | Value | Language |
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dc.contributor.author | 김은정 | - |
dc.contributor.author | 이종민 | - |
dc.contributor.author | 정한성 | - |
dc.date.accessioned | 2014-12-18T08:48:56Z | - |
dc.date.available | 2014-12-18T08:48:56Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1083-3021 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/87003 | - |
dc.description.abstract | To reveal the specific functions of various genes during embryonic development, the manipulation of genes using techniques such as electroporation is of fundamental importance for providing direct evidence concerning function or downstream activation of signaling networks. In vitro embryo culture and electroporation are useful techniques to introduce foreign genes, for developmental biology studies. Among the various mammalian culture techniques, Trowell culture is suitable for studies of embryonic mammary gland development because of its stability and ease of use in conjunction with electroporation technique application. The manipulation of gene expression using electroporation is a useful technique for the functional analysis of a particular gene. In this protocol, full steps for electroporation and in vitro embryo culture have been described for use in embryonic mammary gland development research. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Electroporation/methods* | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Gene Expression Regulation, Developmental* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mammary Glands, Animal/embryology* | - |
dc.subject.MESH | Mammary Glands, Human/embryology* | - |
dc.title | A Method for Electroporation to Study Gene Function in Mammary Gland Development | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Jong-Min Lee | - |
dc.contributor.googleauthor | Eun-Jung Kim | - |
dc.contributor.googleauthor | Han-Sung Jung | - |
dc.identifier.doi | 10.1007/s10911-013-9292-6 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03758 | - |
dc.contributor.localId | A00814 | - |
dc.contributor.localId | A04640 | - |
dc.relation.journalcode | J01569 | - |
dc.identifier.eissn | 1573-7039 | - |
dc.identifier.pmid | 23666358 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs10911-013-9292-6 | - |
dc.subject.keyword | Electroporation | - |
dc.subject.keyword | In vitro embryo culture | - |
dc.subject.keyword | Gene transfer | - |
dc.subject.keyword | Mouse | - |
dc.subject.keyword | Mammary gland | - |
dc.contributor.alternativeName | Kim, Eun Jung | - |
dc.contributor.alternativeName | Lee, Jong Min | - |
dc.contributor.alternativeName | Jung, Han Sung | - |
dc.contributor.affiliatedAuthor | Jung, Han Sung | - |
dc.contributor.affiliatedAuthor | Kim, Eun Jung | - |
dc.contributor.affiliatedAuthor | Lee, Jong Min | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 18 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 233 | - |
dc.citation.endPage | 237 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA, Vol.18(2) : 233-237, 2013 | - |
dc.identifier.rimsid | 32089 | - |
dc.type.rims | ART | - |
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