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Inducing re-epithelialization in skin wound through cultured oral mucosal keratinocytes

 Hyun Sil Kim  ;  Nam Hee Kim  ;  Jin Kim  ;  In Ho Cha 
 Journal of the Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회지), Vol.39(2) : 63-70, 2013 
Journal Title
 Journal of the Korean Association of Oral and Maxillofacial Surgeons  (대한구강악안면외과학회지) 
Issue Date
Objectives The purpose of this study was to investigate the wound healing effect of primary cultured oral mucosal keratinocytes (OMKs) and to assess their roles in skin wounds. Materials and Methods OMK labeled with BromodeoxyUridine were scattered onto 1.5×1.5 cm skin defects of adult female nude mice (OMK group, n=15). For the control, culture media were placed on the wound (control group, n=15). Mice in both groups were sacrificed at three days (n=5), one week (n=5), and two weeks (n=5), and histomorphometric and immunoblot analyses with keratinocyte growth factor (KGF), interleukin (IL)-6, and IL-1α antibody were performed for the biopsied wound specimen. To verify the effect of the cytokine, rhIL-1α was applied instead of OMK transplantation, and the OMK and control groups were compared with regard to re-epithelialization. Results Histomorphometric analyses demonstrated faster re-epithelialization in the graft group than in the control group at the third day, first week, and second week. Newly forming epithelium showed maintenance of the histological character of the skin epithelium. The graft group showed superior expression of KGF, IL-6, and IL-1α protein, compared with the control group. Similar faster re-epithelialization was observed after treatment with rhIL-1α instead of OMK transplantation. Conclusion We successfully confirmed that the graft of primary cultured OMKs promoted regeneration of skin defects. The mechanism of accelerated wound healing by primary cultured OMKs was attributed to inducement of cytokine expression as required for re-epithelialization.
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2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
Yonsei Authors
김남희(Kim, Nam Hee) ORCID logo https://orcid.org/0000-0002-3087-5276
김진(Kim, Jin)
김현실(Kim, Hyun Sil) ORCID logo https://orcid.org/0000-0003-3614-1764
차인호(Cha, In Ho) ORCID logo https://orcid.org/0000-0001-8259-2190
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