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Identifying genes related to radiation resistance in oral squamous cell carcinoma cell lines

DC FieldValueLanguage
dc.contributor.author김세헌-
dc.contributor.author라선영-
dc.contributor.author박행란-
dc.contributor.author조남훈-
dc.contributor.author최윤표-
dc.date.accessioned2014-12-18T08:22:52Z-
dc.date.available2014-12-18T08:22:52Z-
dc.date.issued2013-
dc.identifier.issn0901-5027-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/86199-
dc.description.abstractRadioresistance is one of the main determinants of treatment outcome in oral cancer, but the prediction of radioresistance is difficult. The authors aimed to establish radioresistant oral squamous cell carcinoma (OSCC) cell lines to identify genes with altered expression in response to radioresistance. To induce radioresistant cell lines, the authors treated OSCC cell lines with an accumulated dosage of 60Gy over 30 cycles of radiotherapy. They compared the results from cDNA arrays and proteomics between non-radiated and radioresistant cell lines in order to identify changes in gene expression. Western blot analysis was used to validate the results. The cDNA array revealed 265 commonly up-regulated genes and 268 commonly down-regulated genes in radioresistant cell lines, 30 of which were cancer-related genes. Proteomics identified 51 proteins with commonly altered expression in radioresistant cell lines, 18 of which were cancer-related proteins. Both the cDNA array and proteomics indicated that NM23-H1 and PA2G4 were over-expressed. Western blot analysis showed increased expression of NM23-H1, but not PA2G4, in radioresistant cell lines. The authors concluded that NM23-H1 may be a radioresistance-related gene and over-expression of NM23-H1 could serve as a biomarker to predict radioresistance in OSCC.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfInternational Journal of Oral and Maxillofacial Surgery-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdaptor Proteins, Signal Transducing/genetics-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCarcinoma, Squamous Cell/genetics*-
dc.subject.MESHCarcinoma, Squamous Cell/radiotherapy-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHDNA, Complementary/genetics-
dc.subject.MESHGene Expression Profiling-
dc.subject.MESHGene Expression Regulation, Neoplastic-
dc.subject.MESHHumans-
dc.subject.MESHL-Lactate Dehydrogenase/analysis-
dc.subject.MESHMouth Neoplasms/genetics*-
dc.subject.MESHMouth Neoplasms/radiotherapy-
dc.subject.MESHNM23 Nucleoside Diphosphate Kinases/genetics*-
dc.subject.MESHNeoplasm Proteins/genetics*-
dc.subject.MESHOligonucleotide Array Sequence Analysis-
dc.subject.MESHProteomics-
dc.subject.MESHRNA-Binding Proteins/genetics-
dc.subject.MESHRadiation Tolerance/genetics*-
dc.titleIdentifying genes related to radiation resistance in oral squamous cell carcinoma cell lines-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Otorhinolaryngology (이비인후과학)-
dc.contributor.googleauthorS.Y. Lee-
dc.contributor.googleauthorH.R. Park-
dc.contributor.googleauthorN.H. Cho-
dc.contributor.googleauthorY.P. Choi-
dc.contributor.googleauthorS.Y. Rha-
dc.contributor.googleauthorS.W. Park-
dc.contributor.googleauthorS.-H. Kim-
dc.identifier.doi10.1016/j.ijom.2012.10.022-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00605-
dc.contributor.localIdA01731-
dc.contributor.localIdA03812-
dc.contributor.localIdA04143-
dc.contributor.localIdA01316-
dc.relation.journalcodeJ01143-
dc.identifier.pmid23196067-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0901502712004377-
dc.subject.keywordoral cancer-
dc.subject.keywordradiotherapy-
dc.subject.keywordradioresistance-
dc.subject.keywordgene-
dc.subject.keywordNM23-H1-
dc.contributor.alternativeNameKim, Se Heon-
dc.contributor.alternativeNameRha, Sun Young-
dc.contributor.alternativeNamePark, Haeng Ran-
dc.contributor.alternativeNameCho, Nam Hoon-
dc.contributor.alternativeNameChoi, Yoon Pyo-
dc.contributor.affiliatedAuthorKim, Se Heon-
dc.contributor.affiliatedAuthorPark, Haeng Ran-
dc.contributor.affiliatedAuthorCho, Nam Hoon-
dc.contributor.affiliatedAuthorChoi, Yoon Pyo-
dc.contributor.affiliatedAuthorRha, Sun Young-
dc.rights.accessRightsnot free-
dc.citation.volume42-
dc.citation.number2-
dc.citation.startPage169-
dc.citation.endPage176-
dc.identifier.bibliographicCitationInternational Journal of Oral and Maxillofacial Surgery, Vol.42(2) : 169-176, 2013-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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