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Validation of tumor markers in central nervous system germ cell tumors by real-time reverse transcriptase polymerase chain reaction using formalin-fixed paraffin-embedded tissues

Authors
 Dowhan Kim  ;  Da Hye Lee  ;  Junjeong Choi  ;  Kyu Won Shim  ;  Se Hoon Kim 
Citation
 MOLECULAR MEDICINE REPORTS, Vol.7(1) : 337-341, 2013 
Journal Title
 MOLECULAR MEDICINE REPORTS 
ISSN
 1791-2997 
Issue Date
2013
MeSH
Biomarkers, Tumor/genetics* ; Biomarkers, Tumor/metabolism ; Central Nervous System Neoplasms/diagnosis ; Central Nervous System Neoplasms/genetics* ; Central Nervous System Neoplasms/metabolism ; Humans ; Immunohistochemistry ; Neoplasms, Germ Cell and Embryonal/diagnosis ; Neoplasms, Germ Cell and Embryonal/genetics* ; Neoplasms, Germ Cell and Embryonal/metabolism ; Real-Time Polymerase Chain Reaction ; Reproducibility of Results
Keywords
Biomarkers, Tumor/genetics* ; Biomarkers, Tumor/metabolism ; Central Nervous System Neoplasms/diagnosis ; Central Nervous System Neoplasms/genetics* ; Central Nervous System Neoplasms/metabolism ; Humans ; Immunohistochemistry ; Neoplasms, Germ Cell and Embryonal/diagnosis ; Neoplasms, Germ Cell and Embryonal/genetics* ; Neoplasms, Germ Cell and Embryonal/metabolism ; Real-Time Polymerase Chain Reaction ; Reproducibility of Results
Abstract
The therapeutic protocols for treatment of germinomas and non-germinomatous germ cell tumors (NGGCTs) are completely different, so it is important to distinguish pure germinomas from NGGCTs. As it can be difficult to diagnose by morphology alone, immunohisto-chemistry (IHC) has been widely used as an ancillary test to improve diagnostic accuracy. However, IHC has limitations due to the misinterpretation of results or the aberrant loss of immunoreactivity. However, real-time RT-PCR has certain advantages over IHC, including its quantitative nature. The aim of our study was to evaluate the usefulness of real-time RT-PCR on formalin-fixed paraffin-embedded (FFPE) tissue blocks for the diagnosis of germ cell tumors of the central nervous system. We selected eight markers of germ cell tumors using a literature search, and validated them using real-time RT-PCR. Among them, POU5F1, NANOG and TGFB2 were statistically significant (P=0.05) in multiple comparisons (MANOVA) of three groups (pure germinomas, mature teratomas and malignant germ cell tumors). Two-group (pure germinomas and NGGCTs) discriminant analysis achieved a 70.0% success rate in cross-validation. We concluded that real-time RT-PCR using FFPE tissue has adequate validating power comparable to IHC in the diagnosis of central nervous system germ cell tumors; therefore, when IHC is not available, not conclusive or not informative, RT-PCR is a potential alternative to a repeat biopsy.
Full Text
http://www.spandidos-publications.com/mmr/7/1/337
DOI
10.3892/mmr.2012.1144
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Hoon(김세훈) ORCID logo https://orcid.org/0000-0001-7516-7372
Shim, Kyu Won(심규원) ORCID logo https://orcid.org/0000-0002-9441-7354
Lee, Da Hye(이다혜)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/86181
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