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Proteomics Profiling of Bilirubin Nanoparticle Treatment against Myocardial Ischemia-Reperfusion Injury

Authors
 Soo Jin Kim  ;  Yeseul Park  ;  Yuri Cho  ;  Heeyoun Hwang  ;  Dong Jin Joo  ;  Kyu Ha Huh  ;  Juhan Lee 
Citation
 JOURNAL OF PROTEOME RESEARCH, Vol.23(9) : 3858-3866, 2024-09 
Journal Title
JOURNAL OF PROTEOME RESEARCH
ISSN
 1535-3893 
Issue Date
2024-09
MeSH
Animals ; Bilirubin* / pharmacology ; Chromatography, Liquid ; Disease Models, Animal ; Male ; Myocardial Reperfusion Injury* / drug therapy ; Myocardial Reperfusion Injury* / metabolism ; Nanoparticles* / chemistry ; Proteomics* / methods ; Rats ; Rats, Sprague-Dawley ; Tandem Mass Spectrometry* ; Wnt Signaling Pathway / drug effects
Keywords
bilirubin nanoparticle ; biology/disease-driven human proteome project ; heart ; ischemia-reperfusion injury ; proteomics
Abstract
In myocardial infarction, ischemia-reperfusion injury (IRI) poses a significant challenge due to a lack of effective treatments. Bilirubin, a natural compound known for its anti-inflammatory and antioxidant properties, has been identified as a potential therapeutic agent for IRI. Currently, there are no reports about proteomic studies related to IRI and bilirubin treatment. In this study, we explored the effects of bilirubin nanoparticles in a rat model of myocardial IRI. A total of 3616 protein groups comprising 76,681 distinct peptides were identified using LC-MS/MS, where we distinguished two kinds of protein groups: those showing increased expression in IRI and decreased expression in IRI with bilirubin treatment, and vice versa, accounting for 202 and 35 proteins, respectively. Our proteomic analysis identified significant upregulation in the Wnt and insulin signaling pathways and increased Golgi markers, indicating their role in mediating bilirubin nanoparticle's protective effects. This research contributes to the proteomic understanding of myocardial IRI and suggests bilirubin nanoparticles as a promising strategy for cardiac protection, warranting further investigation in human models.
Full Text
https://pubs.acs.org/doi/10.1021/acs.jproteome.4c00170
DOI
10.1021/acs.jproteome.4c00170
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Soo Jin(김수진)
Lee, Ju Han(이주한)
Cho, Yu Ri(조유리)
Joo, Dong Jin(주동진) ORCID logo https://orcid.org/0000-0001-8405-1531
Huh, Kyu Ha(허규하) ORCID logo https://orcid.org/0000-0003-1364-6989
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/201236
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