Cited 0 times in

Distinct eLPBChAT projections for methamphetamine withdrawal anxiety and primed reinstatement of conditioned place preference

Authors
 Wenwen Chen  ;  Hao Guo  ;  Ning Zhou  ;  Yuning Mai  ;  Tao Hu  ;  Xing Xu  ;  Teng He  ;  Jun Wen  ;  Shan Qin  ;  Chengyong Liu  ;  Wenzhong Wu  ;  Hee Young Kim  ;  Yu Fan  ;  Feifei Ge  ;  Xiaowei Guan 
Citation
 THERANOSTICS, Vol.14(7) : 2881-2896, 2024-04 
Journal Title
THERANOSTICS
Issue Date
2024-04
MeSH
Animals ; Anxiety* / metabolism ; Behavior, Animal / drug effects ; Choline O-Acetyltransferase / metabolism ; Male ; Methamphetamine* / adverse effects ; Mice ; Mice, Inbred C57BL* ; Neurons / metabolism ; Septal Nuclei / metabolism ; Substance Withdrawal Syndrome* / metabolism ; Substance Withdrawal Syndrome* / physiopathology
Keywords
anxiety ; conditioned place preference ; eLPBChAT-lCeAPKCδ ; eLPBChAT-ovBNSTPKCδ ; methamphetamine
Abstract
Methamphetamine (METH) withdrawal anxiety symptom and relapse have been significant challenges for clinical practice, however, the underlying neuronal basis remains unclear. Our recent research has identified a specific subpopulation of choline acetyltransferase (ChAT+) neurons localized in the external lateral portion of parabrachial nucleus (eLPBChAT), which modulates METH primed-reinstatement of conditioned place preference (CPP). Here, the anatomical structures and functional roles of eLPBChAT projections in METH withdrawal anxiety and primed reinstatement were further explored. Methods: In the present study, a multifaceted approach was employed to dissect the LPBChAT+ projections in male mice, including anterograde and retrograde tracing, acetylcholine (Ach) indicator combined with fiber photometry recording, photogenetic and chemogenetic regulation, as well as electrophysiological recording. METH withdrawal anxiety-like behaviors and METH-primed reinstatement of conditioned place preference (CPP) were assessed in male mice. Results: We identified that eLPBChAT send projections to PKCδ-positive (PKCδ+) neurons in lateral portion of central nucleus of amygdala (lCeAPKCδ) and oval portion of bed nucleus of the stria terminalis (ovBNSTPKCδ), forming eLPBChAT-lCeAPKCδ and eLPBChAT-ovBNSTPKCδ pathways. At least in part, the eLPBChAT neurons positively innervate lCeAPKCδ neurons and ovBNSTPKCδ neurons through regulating synaptic elements of presynaptic Ach release and postsynaptic nicotinic acetylcholine receptors (nAChRs). METH withdrawal anxiety and METH-primed reinstatement of CPP respectively recruit eLPBChAT-lCeAPKCδ pathway and eLPBChAT-ovBNSTPKCδ pathway in male mice. Conclusion: Our findings put new insights into the complex neural networks, especially focusing on the eLPBChAT projections. The eLPBChAT is a critical node in the neural networks governing METH withdrawal anxiety and primed-reinstatement of CPP through its projections to the lCeAPKCδ and ovBNSTPKCδ, respectively.
Files in This Item:
T202404720.pdf Download
DOI
10.7150/thno.95383
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hee Young(김희영) ORCID logo https://orcid.org/0000-0002-2495-9115
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/200295
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links