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Designing phage cocktails to combat the emergence of bacteriophage-resistant mutants in multidrug-resistant Klebsiella pneumoniae

Authors
 Seongjun Yoo  ;  Kang-Mu Lee  ;  Nayoung Kim  ;  Thao Nguyen Vu  ;  Ricardo Abadie  ;  Dongeun Yong 
Citation
 MICROBIOLOGY SPECTRUM, Vol.12(1) : e0125823, 2024-01 
Journal Title
MICROBIOLOGY SPECTRUM
Issue Date
2024-01
MeSH
Anti-Bacterial Agents / pharmacology ; Bacteriophages* / genetics ; Host Specificity ; Klebsiella pneumoniae ; Phage Therapy*
Keywords
Klebsiella pneumoniae ; bacteriophage ; phage cocktail ; phage resistance
Abstract
IMPORTANCE: In this study, we aimed to design a novel and effective bacteriophage cocktail that can target both wild-type bacteria and phage-resistant mutants. To achieve this goal, we isolated four phages (U2874, phi_KPN_H2, phi_KPN_S3, and phi_KPN_HS3) that recognized different bacterial surface molecules using phage-resistant bacteria. We constructed three phage cocktails and tested their phage resistance-suppressing ability against multidrug-resistant Klebsiella pneumoniae. We argue that the phage cocktail that induces resensitization of phage susceptibility exhibited superior phage resistance-suppressing ability. Moreover, we observed trade-off effects that manifested progressively in phage-resistant bacteria. We hypothesize that such trade-off effects can augment therapeutic efficacy. We also recommend collating phage host range data against phage-resistant mutants in addition to wild-type bacteria when establishing phage banks to improve the efficiency of phage therapy. Our study underscores the importance of phage host range data in constructing effective phage cocktails for clinical use.
Files in This Item:
T202401002.pdf Download
DOI
10.1128/spectrum.01258-23
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/198607
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