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Appelmans protocol – A directed in vitro evolution enables induction and recombination of prophages with expanded host range

Authors
 Thao Nguyen Vu  ;  Justin Ryan Clark  ;  Eris Jang  ;  Roshan D'Souza  ;  Le Phuong Nguyen  ;  Naina Adren Pinto  ;  Seongjun Yoo  ;  Ricardo Abadie  ;  Anthony William Maresso  ;  Dongeun Yong 
Citation
 VIRUS RESEARCH, Vol.339 : 199272, 2024-01 
Journal Title
VIRUS RESEARCH
ISSN
 0168-1702 
Issue Date
2024-01
MeSH
Bacteriophages* / genetics ; Host Specificity ; Prophages* / genetics ; Recombination, Genetic
Keywords
Acinetobacter baumannii ; Appelmans protocol ; Bacteriophage ; Host range expansion ; Phage therapy ; Prophage induction
Abstract
Infections caused by carbapenem-resistant Acinetobacter baumannii (CRAB) present significant healthcare challenges due to limited treatment options. Bacteriophage (phage) therapy offers potential as an alternative treatment. However, the high host specificity of phages poses challenges for their therapeutic application. To broaden the phage spectrum, laboratory-based phage training using the Appelmans protocol was employed in this study. As a result, the protocol successfully expanded the host range of a phage cocktail targeting CRAB. Further analysis revealed that the expanded host range phages isolated from the output cocktail were identified as recombinant derivatives originating from prophages induced from encountered bacterial strains. These findings provide valuable genetic insights into the protocol's mechanism when applied to phages infecting A. baumannii strains that have never been investigated before. However, it is noteworthy that the expanded host range phages obtained from this protocol exhibited limited stability, raising concerns about their suitability for therapeutic purposes.
Files in This Item:
T202400881.pdf Download
DOI
10.1016/j.virusres.2023.199272
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/198578
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