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The Combination of Mechanically Isolated Stromal Vascular Fraction and Fibrin Hydrogel: A Processing Protocol
DC Field | Value | Language |
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dc.contributor.author | 송승용 | - |
dc.date.accessioned | 2024-03-22T06:16:35Z | - |
dc.date.available | 2024-03-22T06:16:35Z | - |
dc.date.issued | 2023-11 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/198495 | - |
dc.description.abstract | The regenerative potential of adipose-derived stromal cells (ASCs) has gained significant attention in regenerative and translational research. In the past, the extraction of these cells from adipose tissue required a multistep enzyme-based process, resulting in a heterogenous cell mix consisting of ACSs and other cells, which are jointly termed the stromal vascular fraction (SVF). More recently introduced mechanical SVF (mSVF) isolation protocols are less time-consuming and bypass regulatory concerns. We recently proposed a protocol that generates mSVF rich in stromal cells based on a combination of emulsification and centrifugation. One current issue in mSVF application for wound therapy application is the lack of a scaffold providing protection from mechanical manipulation and desiccation. Fibrin hydrogels have been shown to be a useful adjunct in cell transfer for wound healing purposes in the past. In the work herein, we delineate the preparation steps of an mSVF-fibrin hydrogel construct as a novel approach for translational research and clinical application. © 2023 JoVE Journal of Visualized Experiments. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | MYJoVE Corporation | - |
dc.relation.isPartOf | JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Adipose Tissue / blood supply | - |
dc.subject.MESH | Fibrin* | - |
dc.subject.MESH | Hydrogels | - |
dc.subject.MESH | Stromal Cells | - |
dc.subject.MESH | Stromal Vascular Fraction* | - |
dc.title | The Combination of Mechanically Isolated Stromal Vascular Fraction and Fibrin Hydrogel: A Processing Protocol | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Plastic and Reconstructive Surgery (성형외과학교실) | - |
dc.contributor.googleauthor | Pablo Pfister | - |
dc.contributor.googleauthor | Gregory Reid | - |
dc.contributor.googleauthor | Tabea Breckwoldt | - |
dc.contributor.googleauthor | Mauro Vasella | - |
dc.contributor.googleauthor | Ann-Kathrin Seitz | - |
dc.contributor.googleauthor | Seung Yong Song | - |
dc.contributor.googleauthor | Bong-Sung Kim | - |
dc.identifier.doi | 10.3791/65860 | - |
dc.contributor.localId | A02032 | - |
dc.relation.journalcode | J03021 | - |
dc.identifier.eissn | 1940-087X | - |
dc.identifier.pmid | 38047553 | - |
dc.identifier.url | https://app.jove.com/kr/t/65860/the-combination-mechanically-isolated-stromal-vascular-fraction | - |
dc.contributor.alternativeName | Song, Seung Yong | - |
dc.contributor.affiliatedAuthor | 송승용 | - |
dc.citation.volume | 2023 | - |
dc.citation.number | 201 | - |
dc.identifier.bibliographicCitation | JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, Vol.2023(201), 2023-11 | - |
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