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Highly chromophoric dual-terminus labeling of an intrinsically disordered native eukaryotic protein of interest at nanoscale

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dc.contributor.author성백린-
dc.date.accessioned2024-02-15T07:00:22Z-
dc.date.available2024-02-15T07:00:22Z-
dc.date.issued2023-08-
dc.identifier.issn0141-8130-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/198115-
dc.description.abstractChemical conjugation of purified proteins of interest (POIs) in Escherichia coli cells is effective for high expression but has limitations for highly chromogenic dual labeling of intrinsically disordered native proteins (IDNPs). Our probes can tag IDNPs using chemical conjugation during protein synthesis and folding while preserving biologically active structures in mammalian cells. We fluorescently labeled IDNPs in mammalian cells using pure fluorescent methionine and ATTO 565-biotin at the N-or C-terminus, respectively. The dual-labeled Tat protein was used as a model for IDNPs in HeLa cells and detected using Ni-NTA beads to estimate its highly chromogenic concentration. We also demonstrated highly chromogenic double labeling of genetically encoded fluorescent-Tat expression in eukaryotic cells using a single fluorescent dye pair with Fo & BULL;rster resonance energy transfer (FRET) ratio and two-color correlation analysis. This study aims to solve native POI processing and achieve ultrasensitive protein folding for biological and ecological applications at the nanoscale.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier-
dc.relation.isPartOfINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHEscherichia coli / genetics-
dc.subject.MESHEscherichia coli / metabolism-
dc.subject.MESHEukaryota-
dc.subject.MESHEukaryotic Cells*-
dc.subject.MESHFluorescence Resonance Energy Transfer-
dc.subject.MESHFluorescent Dyes / chemistry-
dc.subject.MESHHeLa Cells-
dc.subject.MESHHumans-
dc.subject.MESHIntrinsically Disordered Proteins* / metabolism-
dc.titleHighly chromophoric dual-terminus labeling of an intrinsically disordered native eukaryotic protein of interest at nanoscale-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Microbiology (미생물학교실)-
dc.contributor.googleauthorJung Min Kim-
dc.contributor.googleauthorBaik Lin Seong-
dc.contributor.googleauthorJinho Jung-
dc.identifier.doi10.1016/j.ijbiomac.2023.125396-
dc.contributor.localIdA06211-
dc.relation.journalcodeJ03456-
dc.identifier.eissn1879-0003-
dc.identifier.pmid37348577-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0141813023022900-
dc.subject.keywordBead assay-
dc.subject.keywordDual-fluorescent probe-
dc.subject.keywordEukaryotic protein labeling-
dc.subject.keywordIntrinsically disordered native protein (IDNP). Macromolecular crowding-
dc.subject.keywordTwo-color correlation-
dc.contributor.alternativeNameSeong, Baik L-
dc.contributor.affiliatedAuthor성백린-
dc.citation.volume245-
dc.citation.startPage125396-
dc.identifier.bibliographicCitationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, Vol.245 : 125396, 2023-08-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

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