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Highly chromophoric dual-terminus labeling of an intrinsically disordered native eukaryotic protein of interest at nanoscale
DC Field | Value | Language |
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dc.contributor.author | 성백린 | - |
dc.date.accessioned | 2024-02-15T07:00:22Z | - |
dc.date.available | 2024-02-15T07:00:22Z | - |
dc.date.issued | 2023-08 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/198115 | - |
dc.description.abstract | Chemical conjugation of purified proteins of interest (POIs) in Escherichia coli cells is effective for high expression but has limitations for highly chromogenic dual labeling of intrinsically disordered native proteins (IDNPs). Our probes can tag IDNPs using chemical conjugation during protein synthesis and folding while preserving biologically active structures in mammalian cells. We fluorescently labeled IDNPs in mammalian cells using pure fluorescent methionine and ATTO 565-biotin at the N-or C-terminus, respectively. The dual-labeled Tat protein was used as a model for IDNPs in HeLa cells and detected using Ni-NTA beads to estimate its highly chromogenic concentration. We also demonstrated highly chromogenic double labeling of genetically encoded fluorescent-Tat expression in eukaryotic cells using a single fluorescent dye pair with Fo & BULL;rster resonance energy transfer (FRET) ratio and two-color correlation analysis. This study aims to solve native POI processing and achieve ultrasensitive protein folding for biological and ecological applications at the nanoscale. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Elsevier | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Escherichia coli / genetics | - |
dc.subject.MESH | Escherichia coli / metabolism | - |
dc.subject.MESH | Eukaryota | - |
dc.subject.MESH | Eukaryotic Cells* | - |
dc.subject.MESH | Fluorescence Resonance Energy Transfer | - |
dc.subject.MESH | Fluorescent Dyes / chemistry | - |
dc.subject.MESH | HeLa Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Intrinsically Disordered Proteins* / metabolism | - |
dc.title | Highly chromophoric dual-terminus labeling of an intrinsically disordered native eukaryotic protein of interest at nanoscale | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학교실) | - |
dc.contributor.googleauthor | Jung Min Kim | - |
dc.contributor.googleauthor | Baik Lin Seong | - |
dc.contributor.googleauthor | Jinho Jung | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2023.125396 | - |
dc.contributor.localId | A06211 | - |
dc.relation.journalcode | J03456 | - |
dc.identifier.eissn | 1879-0003 | - |
dc.identifier.pmid | 37348577 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0141813023022900 | - |
dc.subject.keyword | Bead assay | - |
dc.subject.keyword | Dual-fluorescent probe | - |
dc.subject.keyword | Eukaryotic protein labeling | - |
dc.subject.keyword | Intrinsically disordered native protein (IDNP). Macromolecular crowding | - |
dc.subject.keyword | Two-color correlation | - |
dc.contributor.alternativeName | Seong, Baik L | - |
dc.contributor.affiliatedAuthor | 성백린 | - |
dc.citation.volume | 245 | - |
dc.citation.startPage | 125396 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, Vol.245 : 125396, 2023-08 | - |
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