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Charge-shifting polyplex as a viral RNA extraction carrier for streamlined detection of infectious viruses

Authors
 Younseong Song  ;  Jayeon Song  ;  Seongeun Kim  ;  Hyowon Jang  ;  Hogi Kim  ;  Booseok Jeong  ;  Nahyun Park  ;  Sunjoo Kim  ;  Dongeun Yong  ;  Eun-Kyung Lim  ;  Kyoung G Lee  ;  Taejoon Kang  ;  Sung Gap Im 
Citation
 Materials Horizons, Vol.10(10) : 4571-4580, 2023-10 
Journal Title
 Materials Horizons 
Issue Date
2023-10
MeSH
COVID-19 Testing ; COVID-19* / diagnosis ; Humans ; RNA, Viral / analysis ; RNA, Viral / genetics ; SARS-CoV-2* / genetics
Abstract
The recent outbreak of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has highlighted the need for rapid, user-friendly nucleic acid testing that involves simple but efficient RNA extraction. Here, we present a charge-shifting polyplex as an RNA extraction carrier for advanced diagnosis of infectious viral diseases. The polyplex comprises poly(2-(dimethylamino) ethyl acrylate) (pDMAEA) electrostatically conjugated with RNA. The pDMAEA film can rapidly dissolve in the viral RNA solution, promoting immediate binding with RNA to form the polyplex, which enables the efficient capture of a substantial quantity of RNA. Subsequently, the captured RNA can be readily released by the quick hydrolysis of pDMAEA at the onset of quantitative reverse transcription-polymerase chain reaction (qRT-PCR), streamlining the entire process from RNA extraction to analysis. The developed method requires only 5 min of centrifugation and enables the detection of RNA in a one-pot setup. Moreover, the proposed method is fully compatible with high-speed qRT-PCR kits and can identify clinical samples within 1 h including the entire extraction to detection procedure. Indeed, the method successfully detected influenza viruses, SARS-CoV-2, and their delta and omicron variants in 260 clinical samples with a sensitivity of 99.4% and specificity of 98.9%. This rapid, user-friendly polyplex-based approach represents a significant breakthrough in molecular diagnostics.
Full Text
https://pubs.rsc.org/en/content/articlelanding/2023/mh/d3mh00861d
DOI
10.1039/d3mh00861d
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/197911
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