Cited 1 times in
Optimization of the optical transparency of bones by PACT-based passive tissue clearing
DC Field | Value | Language |
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dc.contributor.author | 구성욱 | - |
dc.contributor.author | 박정윤 | - |
dc.contributor.author | 우지원 | - |
dc.contributor.author | 조용은 | - |
dc.contributor.author | 현영민 | - |
dc.contributor.author | 진병호 | - |
dc.date.accessioned | 2023-11-07T07:48:48Z | - |
dc.date.available | 2023-11-07T07:48:48Z | - |
dc.date.issued | 2023-10 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/196523 | - |
dc.description.abstract | Recent developments in tissue clearing methods such as the passive clearing technique (PACT) have allowed three-dimensional analysis of biological structures in whole, intact tissues, thereby providing a greater understanding of spatial relationships and biological circuits. Nonetheless, the issues that remain in maintaining structural integrity and preventing tissue expansion/shrinkage with rapid clearing still inhibit the wide application of these techniques in hard bone tissues, such as femurs and tibias. Here, we present an optimized PACT-based bone-clearing method, Bone-mPACT+, that protects biological structures. Bone-mPACT+ and four different decalcifying procedures were tested for their ability to improve bone tissue clearing efficiency without sacrificing optical transparency; they rendered nearly all types of bone tissues transparent. Both mouse and rat bones were nearly transparent after the clearing process. We also present a further modification, the Bone-mPACT+ Advance protocol, which is specifically optimized for processing the largest and hardest rat bones for easy clearing and imaging using established tissue clearing methods. © 2023. The Author(s). | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Optimization of the optical transparency of bones by PACT-based passive tissue clearing | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Neurosurgery (신경외과학교실) | - |
dc.contributor.googleauthor | Byung-Ho Jin | - |
dc.contributor.googleauthor | Jiwon Woo | - |
dc.contributor.googleauthor | Mirae Lee | - |
dc.contributor.googleauthor | Seockmo Ku | - |
dc.contributor.googleauthor | Hyung Seok Moon | - |
dc.contributor.googleauthor | Seung Jun Ryu | - |
dc.contributor.googleauthor | Young-Min Hyun | - |
dc.contributor.googleauthor | Jeong-Yoon Park | - |
dc.contributor.googleauthor | Sung Uk Kuh | - |
dc.contributor.googleauthor | Yong Eun Cho | - |
dc.identifier.doi | 10.1038/s12276-023-01089-8 | - |
dc.contributor.localId | A00196 | - |
dc.contributor.localId | A01650 | - |
dc.contributor.localId | A06262 | - |
dc.contributor.localId | A03865 | - |
dc.contributor.localId | A04814 | - |
dc.relation.journalcode | J00860 | - |
dc.identifier.eissn | 2092-6413 | - |
dc.identifier.pmid | 37779150 | - |
dc.contributor.alternativeName | Kuh, Sung Uk | - |
dc.contributor.affiliatedAuthor | 구성욱 | - |
dc.contributor.affiliatedAuthor | 박정윤 | - |
dc.contributor.affiliatedAuthor | 우지원 | - |
dc.contributor.affiliatedAuthor | 조용은 | - |
dc.contributor.affiliatedAuthor | 현영민 | - |
dc.citation.startPage | epub | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL AND MOLECULAR MEDICINE : epub, 2023-10 | - |
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