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Promoting angiogenesis and diabetic wound healing through delivery of protein transduction domain-BMP2 formulated nanoparticles with hydrogel

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dc.contributor.author김남희-
dc.contributor.author김현실-
dc.contributor.author박광환-
dc.contributor.author육종인-
dc.contributor.author이경미-
dc.contributor.author이진우-
dc.date.accessioned2023-11-07T07:37:27Z-
dc.date.available2023-11-07T07:37:27Z-
dc.date.issued2023-08-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/196473-
dc.description.abstractDecreased angiogenesis contributes to delayed wound healing in diabetic patients. Recombinant human bone morphogenetic protein-2 (rhBMP2) has also been demonstrated to promote angiogenesis. However, the short half-lives of soluble growth factors, including rhBMP2, limit their use in wound-healing applications. To address this limitation, we propose a novel delivery model using a protein transduction domain (PTD) formulated in a lipid nanoparticle (LNP). We aimed to determine whether a gelatin hydrogel dressing loaded with LNP-formulated PTD-BMP2 (LNP-PTD-BMP2) could enhance the angiogenic function of BMP2 and improve diabetic wound healing. In vitro, compared to the control and rhBMP2, LNP-PTD-BMP2 induced greater tube formation in human umbilical vein endothelial cells and increased the cell recruitment capacity of HaCaT cells. We inflicted large, full-thickness back skin wounds on streptozotocin-induced diabetic mice and applied gelatin hydrogel (GH) cross-linked by microbial transglutaminase containing rhBMP2, LNP-PTD-BMP2, or a control to these wounds. Wounds treated with LNP-PTD-BMP2-loaded GH exhibited enhanced wound closure, increased re-epithelialization rates, and higher collagen deposition than those with other treatments. Moreover, LNP-PTD-BMP2-loaded GH treatment resulted in more CD31- and α-SMA-positive cells, indicating greater neovascularization capacity than rhBMP2-loaded GH or GH treatments alone. Furthermore, in vivo near-infrared fluorescence revealed that LNP-PTD-BMP2 has a longer half-life than rhBMP2 and that BMP2 localizes around wounds. In conclusion, LNP-PTD-BMP2-loaded GH is a viable treatment option for diabetic wounds.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherSage-
dc.relation.isPartOfJOURNAL OF TISSUE ENGINEERING-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titlePromoting angiogenesis and diabetic wound healing through delivery of protein transduction domain-BMP2 formulated nanoparticles with hydrogel-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentResearch Institute (부설연구소)-
dc.contributor.googleauthorJae Wan Suh-
dc.contributor.googleauthorKyoung-Mi Lee-
dc.contributor.googleauthorEun Ae Ko-
dc.contributor.googleauthorDong Suk Yoon-
dc.contributor.googleauthorKwang Hwan Park-
dc.contributor.googleauthorHyun Sil Kim-
dc.contributor.googleauthorJong In Yook-
dc.contributor.googleauthorNam Hee Kim-
dc.contributor.googleauthorJin Woo Lee-
dc.identifier.doi10.1177/20417314231190641-
dc.contributor.localIdA00360-
dc.contributor.localIdA01121-
dc.contributor.localIdA01437-
dc.contributor.localIdA02536-
dc.contributor.localIdA04619-
dc.contributor.localIdA03230-
dc.relation.journalcodeJ04010-
dc.identifier.eissn2041-7314-
dc.identifier.pmid37601810-
dc.subject.keywordDiabetic wound healing-
dc.subject.keywordangiogenesis-
dc.subject.keywordbone morphogenetic protein-2-
dc.subject.keywordlipid nanoparticle-
dc.subject.keywordprotein transduction domain-
dc.contributor.alternativeNameKim, Nam Hee-
dc.contributor.affiliatedAuthor김남희-
dc.contributor.affiliatedAuthor김현실-
dc.contributor.affiliatedAuthor박광환-
dc.contributor.affiliatedAuthor육종인-
dc.contributor.affiliatedAuthor이경미-
dc.contributor.affiliatedAuthor이진우-
dc.citation.volume14-
dc.identifier.bibliographicCitationJOURNAL OF TISSUE ENGINEERING, Vol.14, 2023-08-
Appears in Collections:
2. College of Dentistry (치과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers

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