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Promoting angiogenesis and diabetic wound healing through delivery of protein transduction domain-BMP2 formulated nanoparticles with hydrogel
DC Field | Value | Language |
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dc.contributor.author | 김남희 | - |
dc.contributor.author | 김현실 | - |
dc.contributor.author | 박광환 | - |
dc.contributor.author | 육종인 | - |
dc.contributor.author | 이경미 | - |
dc.contributor.author | 이진우 | - |
dc.date.accessioned | 2023-11-07T07:37:27Z | - |
dc.date.available | 2023-11-07T07:37:27Z | - |
dc.date.issued | 2023-08 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/196473 | - |
dc.description.abstract | Decreased angiogenesis contributes to delayed wound healing in diabetic patients. Recombinant human bone morphogenetic protein-2 (rhBMP2) has also been demonstrated to promote angiogenesis. However, the short half-lives of soluble growth factors, including rhBMP2, limit their use in wound-healing applications. To address this limitation, we propose a novel delivery model using a protein transduction domain (PTD) formulated in a lipid nanoparticle (LNP). We aimed to determine whether a gelatin hydrogel dressing loaded with LNP-formulated PTD-BMP2 (LNP-PTD-BMP2) could enhance the angiogenic function of BMP2 and improve diabetic wound healing. In vitro, compared to the control and rhBMP2, LNP-PTD-BMP2 induced greater tube formation in human umbilical vein endothelial cells and increased the cell recruitment capacity of HaCaT cells. We inflicted large, full-thickness back skin wounds on streptozotocin-induced diabetic mice and applied gelatin hydrogel (GH) cross-linked by microbial transglutaminase containing rhBMP2, LNP-PTD-BMP2, or a control to these wounds. Wounds treated with LNP-PTD-BMP2-loaded GH exhibited enhanced wound closure, increased re-epithelialization rates, and higher collagen deposition than those with other treatments. Moreover, LNP-PTD-BMP2-loaded GH treatment resulted in more CD31- and α-SMA-positive cells, indicating greater neovascularization capacity than rhBMP2-loaded GH or GH treatments alone. Furthermore, in vivo near-infrared fluorescence revealed that LNP-PTD-BMP2 has a longer half-life than rhBMP2 and that BMP2 localizes around wounds. In conclusion, LNP-PTD-BMP2-loaded GH is a viable treatment option for diabetic wounds. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Sage | - |
dc.relation.isPartOf | JOURNAL OF TISSUE ENGINEERING | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Promoting angiogenesis and diabetic wound healing through delivery of protein transduction domain-BMP2 formulated nanoparticles with hydrogel | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Research Institute (부설연구소) | - |
dc.contributor.googleauthor | Jae Wan Suh | - |
dc.contributor.googleauthor | Kyoung-Mi Lee | - |
dc.contributor.googleauthor | Eun Ae Ko | - |
dc.contributor.googleauthor | Dong Suk Yoon | - |
dc.contributor.googleauthor | Kwang Hwan Park | - |
dc.contributor.googleauthor | Hyun Sil Kim | - |
dc.contributor.googleauthor | Jong In Yook | - |
dc.contributor.googleauthor | Nam Hee Kim | - |
dc.contributor.googleauthor | Jin Woo Lee | - |
dc.identifier.doi | 10.1177/20417314231190641 | - |
dc.contributor.localId | A00360 | - |
dc.contributor.localId | A01121 | - |
dc.contributor.localId | A01437 | - |
dc.contributor.localId | A02536 | - |
dc.contributor.localId | A04619 | - |
dc.contributor.localId | A03230 | - |
dc.relation.journalcode | J04010 | - |
dc.identifier.eissn | 2041-7314 | - |
dc.identifier.pmid | 37601810 | - |
dc.subject.keyword | Diabetic wound healing | - |
dc.subject.keyword | angiogenesis | - |
dc.subject.keyword | bone morphogenetic protein-2 | - |
dc.subject.keyword | lipid nanoparticle | - |
dc.subject.keyword | protein transduction domain | - |
dc.contributor.alternativeName | Kim, Nam Hee | - |
dc.contributor.affiliatedAuthor | 김남희 | - |
dc.contributor.affiliatedAuthor | 김현실 | - |
dc.contributor.affiliatedAuthor | 박광환 | - |
dc.contributor.affiliatedAuthor | 육종인 | - |
dc.contributor.affiliatedAuthor | 이경미 | - |
dc.contributor.affiliatedAuthor | 이진우 | - |
dc.citation.volume | 14 | - |
dc.identifier.bibliographicCitation | JOURNAL OF TISSUE ENGINEERING, Vol.14, 2023-08 | - |
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