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Anti-biofilm activity of a novel nanoemulsion containing Curcuma xanthorrhiza oil
DC Field | Value | Language |
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dc.contributor.author | 김백일 | - |
dc.contributor.author | 이은송 | - |
dc.contributor.author | 정회인 | - |
dc.date.accessioned | 2023-10-19T06:06:35Z | - |
dc.date.available | 2023-10-19T06:06:35Z | - |
dc.date.issued | 2023-08 | - |
dc.identifier.issn | 0300-5712 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/196358 | - |
dc.description.abstract | Objectives: We aimed to solubilize Curcuma xanthorrhiza oil (CXO) using nanoemulsification and evaluate its inhibitory effects against biofilm formation. Methods: The components of CXO were evaluated through high-performance liquid chromatography (HPLC) analysis. Healthy human saliva was inoculated onto hydroxyapatite discs to form microcosm biofilms for four days and treated six times with each antimicrobial agent: distilled water (DW), CXO emulsion (EM), CXO nanoemulsion (NE), and positive controls (Listerine and chlorhexidine). Biofilm fluorescence imaging was performed using quantitative light-induced fluorescence, and cell viability and dry-weight measurements were obtained. We compared the bacterial cell and extracellular polysaccharide (EPS) biovolume and thickness using confocal laser scanning microscopy (CLSM). Results: HPLC analysis revealed that CXO was composed of approximately 47% xanthorrhizol. Compared with DW, NE exhibited significantly lower red fluorescence intensity and area (42% and 37%, p < 0.001 and p < 0.001, respectively), and reduced total and aciduric bacterial cell viability (7.3% and 3.9%, p < 0.001, p = 0.01, respectively). Furthermore, the bacterial cell and EPS biovolume and thickness in NE decreased by 40-80% compared to DW, similar to chlorhexidine. Conversely, EM showed a significant difference only in cell viability against total bacteria when compared with DW (p = 0.003), with EPS biovolume and thickness exhibiting higher values than DW. Conclusions: Nanoemulsification successfully solubilized CXO and demonstrated superior anti-biofilm effects compared to the emulsion form. Clinical significance: These findings suggest the potential use of NE as a novel antimicrobial agent for preventing oral diseases. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Elsevier | - |
dc.relation.isPartOf | JOURNAL OF DENTISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Anti-Infective Agents* / pharmacology | - |
dc.subject.MESH | Bacteria | - |
dc.subject.MESH | Biofilms | - |
dc.subject.MESH | Chlorhexidine / pharmacology | - |
dc.subject.MESH | Curcuma | - |
dc.subject.MESH | Drinking Water* | - |
dc.subject.MESH | Emulsions / pharmacology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Saliva / microbiology | - |
dc.title | Anti-biofilm activity of a novel nanoemulsion containing Curcuma xanthorrhiza oil | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Preventive Dentistry and Public Oral Health (예방치과학교실) | - |
dc.contributor.googleauthor | Mu-Yeol Cho | - |
dc.contributor.googleauthor | Eun-Song Lee | - |
dc.contributor.googleauthor | Hoi-In Jung | - |
dc.contributor.googleauthor | Baek-Il Kim | - |
dc.identifier.doi | 10.1016/j.jdent.2023.104647 | - |
dc.contributor.localId | A00485 | - |
dc.contributor.localId | A04635 | - |
dc.contributor.localId | A03788 | - |
dc.relation.journalcode | J01368 | - |
dc.identifier.eissn | 1879-176X | - |
dc.identifier.pmid | 37536430 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0300571223002336 | - |
dc.subject.keyword | Anti-biofilm | - |
dc.subject.keyword | Curcuma xanthorrhiza oil | - |
dc.subject.keyword | Nanoemulsion | - |
dc.contributor.alternativeName | Kim, Baek Il | - |
dc.contributor.affiliatedAuthor | 김백일 | - |
dc.contributor.affiliatedAuthor | 이은송 | - |
dc.contributor.affiliatedAuthor | 정회인 | - |
dc.citation.volume | 137 | - |
dc.citation.startPage | 104647 | - |
dc.identifier.bibliographicCitation | JOURNAL OF DENTISTRY, Vol.137 : 104647, 2023-08 | - |
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