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Bisphenol A release from commercially available 3-dimensionally printed resins and human cell apoptosis to bisphenol A: an in-vitro study

Authors
 Yun Sun Jung  ;  Sang Tae Ro  ;  Sang Wook Kang  ;  Hyeonjong Lee  ;  Jang Sun Lee  ;  Yong Kwon Chae  ;  Ko Eun Lee  ;  Hyo-Seol Lee  ;  Kyu Hwan Kwack  ;  Su Kang Kim  ;  Sung Chul Choi  ;  Ok Hyung Nam 
Citation
 JOURNAL OF CLINICAL PEDIATRIC DENTISTRY, Vol.47(3) : 89-95, 2023-03 
Journal Title
JOURNAL OF CLINICAL PEDIATRIC DENTISTRY
ISSN
 1053-4628 
Issue Date
2023-03
MeSH
Apoptosis ; Benzhydryl Compounds / analysis ; Benzhydryl Compounds / chemistry ; Benzhydryl Compounds / toxicity ; Child ; Composite Resins / chemistry ; Dental Materials* / chemistry ; Humans ; Materials Testing ; Phenols* / analysis ; Phenols* / chemistry ; Phenols* / pharmacology
Keywords
3-dimensional printing ; Bisphenol A ; Children ; Dental materials ; Digital dentistry ; Pediatric dentistry
Abstract
Bisphenol A (BPA) from dental materials may be linked to children's health issues. This study aimed to assess the release of BPA from commercially available 3-dimensional (3D)-printed resin materials and evaluate BPA-related apoptotic effects on human periodontal ligament cells and gingival fibroblasts. Commercially available 3D-printed resin materials for prosthodontic use were selected as follows: NextDent C&B MFH (3D Systems, Rock Hill, SC, USA), DIOnavi-P. MAX (Dio Co., Busan, Korea), and DIOnavi-Denture02 (Dio Co., Busan, Korea). Identical cuboidal samples (1 cm × 1 cm × 0.5 cm) were printed from the materials and cured. BPA release was assessed using liquid chromatography/mass spectrometry (LC/MS). In addition, human gingival fibroblasts and periodontal ligament cells were exposed to various BPA solutions based on the LC/MS results. Cell Counting kit-8 (CCK-8) and real-time polymerase chain reaction analyses were performed to evaluate BPA-related apoptotic effects. The LC/MS analysis confirmed that none of the 3D-printed resin materials released BPA after curing. Both human gingival fibroblasts and periodontal ligament cells showed lower viability after BPA exposure. Regarding apoptosis-related gene expression, Caspase10 (CASP10) expression in periodontal ligament cells was significantly different in the BPA solutions (p < 0.05). The expression of BAX and Capspase8 (CASP8) in gingival fibroblasts was significantly increased by BPA in a dose-dependent manner (p < 0.05). Within the limitations of this study, the 3D-printed resin materials were not found to release BPA. This finding implies that 3D-printed resin materials are not associated with potential BPA-related risks in children.
Files in This Item:
T202303771.pdf Download
DOI
10.22514/jocpd.2023.027
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Prosthodontics (보철과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Hyeonjong(이현종) ORCID logo https://orcid.org/0000-0002-1669-2975
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/195573
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