Cited 0 times in
Global loss of cellular m6A RNA methylation following infection with different SARS-CoV-2 variants
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 동재준 | - |
dc.contributor.author | 조재용 | - |
dc.date.accessioned | 2023-07-12T02:29:48Z | - |
dc.date.available | 2023-07-12T02:29:48Z | - |
dc.date.issued | 2023-03 | - |
dc.identifier.issn | 1088-9051 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/195325 | - |
dc.description.abstract | Insights into host–virus interactions during SARS-CoV-2 infection are needed to understand COVID-19 pathogenesis and may help to guide the design of novel antiviral therapeutics. N6-Methyladenosine modification (m6A), one of the most abundant cellular RNA modifications, regulates key processes in RNA metabolism during stress response. Gene expression profiles observed postinfection with different SARS-CoV-2 variants show changes in the expression of genes related to RNA catabolism, including m6A readers and erasers. We found that infection with SARS-CoV-2 variants causes a loss of m6A in cellular RNAs, whereas m6A is detected abundantly in viral RNA. METTL3, the m6A methyltransferase, shows an unusual cytoplasmic localization postinfection. The B.1.351 variant has a less-pronounced effect on METTL3 localization and loss of m6A than did the B.1 and B.1.1.7 variants. We also observed a loss of m6A upon SARS-CoV-2 infection in air/liquid interface cultures of human airway epithelia, confirming that m6A loss is characteristic of SARS-CoV-2-infected cells. Further, transcripts with m6A modification are preferentially down-regulated postinfection. Inhibition of the export protein XPO1 results in the restoration of METTL3 localization, recovery of m6A on cellular RNA, and increased mRNA expression. Stress granule formation, which is compromised by SARS-CoV-2 infection, is restored by XPO1 inhibition and accompanied by a reduced viral infection in vitro. Together, our study elucidates how SARS-CoV-2 inhibits the stress response and perturbs cellular gene expression in an m6A-dependent manner. © 2023 Vaid et al.; Published by Cold Spring Harbor Laboratory Press. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Cold Spring Harbor Laboratory Press | - |
dc.relation.isPartOf | GENOME RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | COVID-19* / genetics | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Methylation | - |
dc.subject.MESH | Methyltransferases / genetics | - |
dc.subject.MESH | RNA | - |
dc.subject.MESH | RNA, Viral / genetics | - |
dc.subject.MESH | SARS-CoV-2* / genetics | - |
dc.title | Global loss of cellular m6A RNA methylation following infection with different SARS-CoV-2 variants | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Family Medicine (가정의학교실) | - |
dc.contributor.googleauthor | Roshan Vaid | - |
dc.contributor.googleauthor | Akram Mendez | - |
dc.contributor.googleauthor | Ketan Thombare | - |
dc.contributor.googleauthor | Rebeca Burgos-Panadero | - |
dc.contributor.googleauthor | Rémy Robinot | - |
dc.contributor.googleauthor | Barbara F Fonseca | - |
dc.contributor.googleauthor | Nikhil R Gandasi | - |
dc.contributor.googleauthor | Johan Ringlander | - |
dc.contributor.googleauthor | Mohammad Hassan Baig | - |
dc.contributor.googleauthor | Jae-June Dong | - |
dc.contributor.googleauthor | Jae Yong Cho | - |
dc.contributor.googleauthor | Björn Reinius | - |
dc.contributor.googleauthor | Lisa A Chakrabarti | - |
dc.contributor.googleauthor | Kristina Nystrom | - |
dc.contributor.googleauthor | Tanmoy Mondal | - |
dc.identifier.doi | 10.1101/gr.276407.121 | - |
dc.contributor.localId | A04927 | - |
dc.contributor.localId | A03899 | - |
dc.relation.journalcode | J03144 | - |
dc.identifier.eissn | 1549-5469 | - |
dc.identifier.pmid | 36859333 | - |
dc.contributor.alternativeName | Dong, Jae June | - |
dc.contributor.affiliatedAuthor | 동재준 | - |
dc.contributor.affiliatedAuthor | 조재용 | - |
dc.citation.volume | 33 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 299 | - |
dc.citation.endPage | 313 | - |
dc.identifier.bibliographicCitation | GENOME RESEARCH, Vol.33(3) : 299-313, 2023-03 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.