골육종에서 p53단백발현에 대한 면역조직화학적 연구

Abstract

p53 gene located in the 17p chromosome have been known as a tumor suppressor gene. But mutant p53 gene is also known to be related to tumorigenesis through the effect on cellular proliferation and differentiation. Although the relation between the mutant p53 gene and the development of osteosarcoma have been studied at the genetic level, the immunohistochemical studies for p53 protein expression in osteosarcoma were very rare. We investigated the rate and degree of aberrant p53 expression, and its relation to each subtype of osteosarcoma. Twenty four cases of osteosarcoma were histologically reviewed and the immunohistochemical stain for p53 protein using D07(Novocastra, Newcastle, UK) were performed in paraffin blocks. Histological review show 11 osteoblastic, 7 fibroblastic, 4 chodroblastic and 2 epithelioid types. Nine(37.5%) out of 24 cases were positive for p53 protein. These consisted of 5 osteoblastic(55.6%), 2 chondroblastic(22.2%), 1 fibroblastic(l1.1 %), and 1 epithelioid(11.1%) types. Diffuse strong positive reaction was occured in 5 out of 9 positive cases and the remaining were focal positive. In conclusion, although the p53 expression rate of osteosarcoma was lower than other neoplasms, the intensity of the immunohistochemical stain was diffuse strong.